ChEMBL ligand: CHEMBL1672380 (1-Adamantanecarbonyl-RF-NH2)
MRGPRX1/Mas-related G-protein coupled receptor member X1 in Human [ChEMBL: CHEMBL5850] [GtoPdb: 156] [UniProtKB: Q96LB2] Mas-related G-protein coupled receptor member X1 in Mouse [ChEMBL: CHEMBL3341576] [UniProtKB: Q8CIP3] Mas-related G-protein coupled receptor member X1 in Rat [ChEMBL: CHEMBL3341575] [UniProtKB: Q8R4G1] There should be some charts here, you may need to enable JavaScript!
kisspeptin receptor/Metastin receptor in Human [ChEMBL: CHEMBL5413] [GtoPdb: 266] [UniProtKB: Q969F8] There should be some charts here, you may need to enable JavaScript!
NPFF1 receptor/Neuropeptide FF receptor 1 in Human [ChEMBL: CHEMBL5951] [GtoPdb: 300] [UniProtKB: Q9GZQ6] There should be some charts here, you may need to enable JavaScript!
NPFF2 receptor/Neuropeptide FF receptor 2 in Human [ChEMBL: CHEMBL5952] [GtoPdb: 301] [UniProtKB: Q9Y5X5] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
MRGPRX1/Mas-related G-protein coupled receptor member X1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5850] [GtoPdb: 156] [UniProtKB: Q96LB2]
ChEMBL	Agonist activity at human MrgX1 transfected in HEK293 cells by FLIPR assay	B	4	pEC50	>100000	nM	EC50	Bioorg Med Chem (2014) 22: 5831-5837 [PMID:25288495]
Mas-related G-protein coupled receptor member X1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3341576] [UniProtKB: Q8CIP3]
ChEMBL	Agonist activity at mouse MrgC11 transfected in HEK293 cells by FLIPR assay	B	6.5	pEC50	316.23	nM	EC50	Bioorg Med Chem (2014) 22: 5831-5837 [PMID:25288495]
Mas-related G-protein coupled receptor member X1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3341575] [UniProtKB: Q8R4G1]
ChEMBL	Agonist activity at rat MrgX1 transfected in HEK293 cells by FLIPR assay	B	5.2	pEC50	6309.57	nM	EC50	Bioorg Med Chem (2014) 22: 5831-5837 [PMID:25288495]
kisspeptin receptor/Metastin receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5413] [GtoPdb: 266] [UniProtKB: Q969F8]
ChEMBL	Displacement of [125I]kisspeptin-15 from GPR54	B	6.08	pIC50	830	nM	IC50	ACS Med Chem Lett (2011) 2: 53-57 [PMID:24900254]
NPFF1 receptor/Neuropeptide FF receptor 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5951] [GtoPdb: 300] [UniProtKB: Q9GZQ6]
GtoPdb	-	-	7.2	pKi	-	-	-	Proc Natl Acad Sci USA (2006) 103: 466-71 [PMID:16407169]
ChEMBL	Displacement of [125I]YVP from human NPFFR1 expressed in CHO cells	B	7.24	pKi	58	nM	Ki	J Med Chem (2020) 63: 12387-12402 [PMID:32673481]
ChEMBL	Displacement of [3H]FFRF-NH2 from human flag-tagged NPFF1 receptor expressed in CHO cells by scintillation counting	B	7.66	pKi	22	nM	Ki	Bioorg Med Chem Lett (2012) 22: 7471-7474 [PMID:23131340]
ChEMBL	Displacement of [125I]-1DMeNPFF from recombinant human NPFF1 receptor expressed in CHO cells by TopCount scintillation counting method	B	7.85	pKi	14	nM	Ki	J Med Chem (2021) 64: 7555-7564 [PMID:34008968]
ChEMBL	Antagonist activity at NPFF1 receptor (unknown origin) expressed in HEK293A cells assessed as reversal of NPPF induced inhibition of forskolin-stimulated cAMP accumulation measured after 30 mins	F	6.39	pIC50	410	nM	IC50	Bioorg Med Chem (2019) 27: 630-643 [PMID:30626554]
ChEMBL	Antagonist activity at human NPFFR1 expressed in CHO cells assessed as reversal of NPVF-induced inhibition of forskolin-stimulated cAMP accumulation incubated for 10 mins by liquid scintillation counting method	F	5.33	pEC50	4700	nM	EC50	J Med Chem (2020) 63: 12387-12402 [PMID:32673481]
ChEMBL	Agonist activity at human NPFF1 receptor F/Y7.35A mutant assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting	F	6.34	pEC50	462	nM	EC50	J Med Chem (2012) 55: 6124-6136 [PMID:22708927]
ChEMBL	Agonist activity at human NPFF1 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting	F	7.15	pEC50	71	nM	EC50	J Med Chem (2012) 55: 6124-6136 [PMID:22708927]
NPFF2 receptor/Neuropeptide FF receptor 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5952] [GtoPdb: 301] [UniProtKB: Q9Y5X5]
GtoPdb	-	-	7.1	pKi	-	-	-	Proc Natl Acad Sci USA (2006) 103: 466-71 [PMID:16407169]
ChEMBL	Displacement of [125I]Tyr-NPFF from human NPFFR2 expressed in green monkey Cos-1 cells	B	7.12	pKi	75	nM	Ki	J Med Chem (2020) 63: 12387-12402 [PMID:32673481]
ChEMBL	Displacement of [3H]FFRF-NH2 from human flag-tagged NPFF2 receptor expressed in CHO cells by scintillation counting	B	7.37	pKi	43	nM	Ki	Bioorg Med Chem Lett (2012) 22: 7471-7474 [PMID:23131340]
ChEMBL	Displacement of [125I]-1DMeNPFF from recombinant human NPFF2 receptor expressed in CHO cells by TopCount scintillation counting method	B	7.74	pKi	18	nM	Ki	J Med Chem (2021) 64: 7555-7564 [PMID:34008968]
ChEMBL	Antagonist activity at NPFF2 receptor (unknown origin) expressed in HEK293A cells assessed as reversal of NPPF induced inhibition of forskolin-stimulated cAMP accumulation measured after 30 mins	F	6.39	pIC50	410	nM	IC50	Bioorg Med Chem (2019) 27: 630-643 [PMID:30626554]
ChEMBL	Agonist activity at human NPFF2 receptor F/Y7.35A mutant assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting	F	6.33	pEC50	472	nM	EC50	J Med Chem (2012) 55: 6124-6136 [PMID:22708927]
ChEMBL	Agonist activity at human NPFF2 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting	F	6.42	pEC50	379	nM	EC50	J Med Chem (2012) 55: 6124-6136 [PMID:22708927]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]