ChEMBL ligand: CHEMBL3392741
ubiquitin specific peptidase 2/Ubiquitin carboxyl-terminal hydrolase 2 in Human [ChEMBL: CHEMBL1293227] [GtoPdb: 2430] [UniProtKB: O75604] There should be some charts here, you may need to enable JavaScript!
ubiquitin specific peptidase 8/Ubiquitin carboxyl-terminal hydrolase 8 in Human [ChEMBL: CHEMBL2157854] [GtoPdb: 3209] [UniProtKB: P40818] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
ubiquitin specific peptidase 2/Ubiquitin carboxyl-terminal hydrolase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1293227] [GtoPdb: 2430] [UniProtKB: O75604]
GtoPdb	Binding affinity for hUSP2	-	5.28	pKd	5200	nM	Kd	Sci Transl Med (2023) 15: eadh7668 [PMID:38055802]
ChEMBL	Inhibition of USP2 (267 to 599 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells using ubiquitin-rhodamine-110 as substrate preincubated with compound for 30 mins followed by substrate addition measured after 30 mins by fluorometric assay	B	5.44	pIC50	3600	nM	IC50	Eur J Med Chem (2024) 268: 116275-116275 [PMID:38452725]
ChEMBL	Inhibition of human USP2 using Di-Ub IQF as substrate incubated for 15 mins by K63-2 assay	B	5.96	pIC50	1100	nM	IC50	Eur J Med Chem (2020) 191: 112107-112107 [PMID:32092586]
ChEMBL	Inhibition of USP2 (unknown origin) using Di-Ub IQF K48-4 as substrate preincubated for 10 mins followed by substrate addition by high-throughput assay	B	5.96	pIC50	1100	nM	IC50	Eur J Med Chem (2024) 268: 116275-116275 [PMID:38452725]
GtoPdb	Inhibition of USP2 de-ubiquitinating activity in vitro	-	5.96	pIC50	1100	nM	IC50	Sci Transl Med (2023) 15: eadh7668 [PMID:38055802]
ubiquitin specific peptidase 8/Ubiquitin carboxyl-terminal hydrolase 8 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2157854] [GtoPdb: 3209] [UniProtKB: P40818]
ChEMBL	Inhibition of N-terminal His6 tagged USP8 (734 to 1110 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells using ubiquitin-rhodamine-110 as substrate preincubated with compound for 30 mins followed by substrate addition measured after 30 mins by fluorometric assay	B	5.33	pIC50	4700	nM	IC50	Eur J Med Chem (2024) 268: 116275-116275 [PMID:38452725]
ChEMBL	Inhibition of human USP8 using Di-Ub IQF as substrate incubated for 15 mins by K63-2 assay	B	6.02	pIC50	950	nM	IC50	Eur J Med Chem (2020) 191: 112107-112107 [PMID:32092586]
ChEMBL	Inhibition of USP8 (unknown origin) using Di-Ub IQF K48-02 as substrate preincubated for 30 mins followed by substrate addition by high-throughput assay	B	6.02	pIC50	950	nM	IC50	Eur J Med Chem (2024) 268: 116275-116275 [PMID:38452725]

ChEMBL data shown on this page come from version 36:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]