ChEMBL ligand: CHEMBL4633662
BLM RecQ like helicase/Bloom syndrome protein in Human [ChEMBL: CHEMBL1293237] [GtoPdb: 3260] [UniProtKB: P54132] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
BLM RecQ like helicase/Bloom syndrome protein in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1293237] [GtoPdb: 3260] [UniProtKB: P54132]
ChEMBL	Binding affinity to His-tagged BLM I1168A mutant (unknown origin) expressed in Escherichia coli BL21 (DE3) cells by isothermal calorimetric titration assay	B	5.18	pKd	6540	nM	Kd	J Med Chem (2020) 63: 9752-9772 [PMID:32697083]
ChEMBL	Binding affinity to His-tagged BLM H996A mutant (unknown origin) expressed in Escherichia coli BL21 (DE3) cells by isothermal calorimetric titration assay	B	5.85	pKd	1400	nM	Kd	J Med Chem (2020) 63: 9752-9772 [PMID:32697083]
ChEMBL	Binding affinity to wild-type His-tagged BLM (642 to 1296 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells by isothermal calorimetric titration assay	B	6.12	pKd	760	nM	Kd	J Med Chem (2020) 63: 9752-9772 [PMID:32697083]
GtoPdb	Binding affinity for wild-type BLM (642-1296) expressed in Escherichia coli, setermined by isothermal calorimetric titration (ICT) assay.	-	6.12	pKd	760	nM	Kd	J Med Chem (2020) 63: 9752-9772 [PMID:32697083]
ChEMBL	Inhibition of BLM (unknown origin) assessed as ATP hydrolysis using 5'-biotin-labeled duplex forked-DNA as substrate incubated for 10 mins in presence of ATP by luciferase assay	B	4.49	pIC50	>32000	nM	IC50	Eur J Med Chem (2023) 246: 114944-114944 [PMID:36459756]
ChEMBL	Displacement of thiazole orange from His-tagged BLM (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as decrease in fluorescence intensity by fluorescence based analysis	B	5	pIC50	>10000	nM	IC50	Eur J Med Chem (2023) 246: 114944-114944 [PMID:36459756]
ChEMBL	Binding affinity to His-tagged BLM (642 to 1296 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells assessed as inhibition of protein interaction with 5'-biotin-labeled forked DNA measured after 1 hr by ELISA	B	5.06	pIC50	8700	nM	IC50	J Med Chem (2020) 63: 9752-9772 [PMID:32697083]
ChEMBL	Inhibition of His-tagged BLM (unknown origin) expressed in Escherichia coli BL21 (DE3) using 5'-biotin-labeled duplex forked-DNA as substrate incubated for 10 mins followed by substrate addition measured after 30 mins in presence of ATP by electrophoretic mobility shift assay	B	5.29	pIC50	5100	nM	IC50	Eur J Med Chem (2023) 246: 114944-114944 [PMID:36459756]
ChEMBL	Inhibition of His-tagged BLM (642 to 1296 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells assessed as reduction in DNA unwinding activity using 5'-biotin-labeled forked DNA as substrate preincubated for 1 hr followed by substrate addition and measured after 1 hr by electrophoretic mobility shift assay	B	6.1	pIC50	800	nM	IC50	J Med Chem (2020) 63: 9752-9772 [PMID:32697083]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]