ChEMBL ligand: CHEMBL4277689
neuraminidase 1/Sialidase 1 in Human [ChEMBL: CHEMBL2726] [GtoPdb: 3214] [UniProtKB: Q99519] There should be some charts here, you may need to enable JavaScript!
neuraminidase 2/Sialidase 2 in Human [ChEMBL: CHEMBL3200] [GtoPdb: 3258] [UniProtKB: Q9Y3R4] There should be some charts here, you may need to enable JavaScript!
Sialidase 4 in Human [ChEMBL: CHEMBL4174] [UniProtKB: Q8WWR8] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
neuraminidase 1/Sialidase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2726] [GtoPdb: 3214] [UniProtKB: Q99519]
GtoPdb	-	-	4.85	pIC50	14000	nM	IC50	J Med Chem (2018) 61: 11261-11279 [PMID:30457869]
ChEMBL	Inhibition of human His6-tagged NEU1 expressed in HEK293 cells using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition and measured after 30 mins by fluorescence assay	B	4.85	pIC50	14000	nM	IC50	J Med Chem (2018) 61: 11261-11279 [PMID:30457869]
ChEMBL	Inhibition of His6-tagged human neuraminidase-1 expressed in HEK293 cells pre-incubated for 15 mins before 4MU-NANA substrate addition and measured after 30 mins by fluorescence based assay	B	4.85	pIC50	14000	nM	IC50	WO-2018213933-A1. Methods of preventing or treating atherosclerosis with inhibitors of specific isoenzymes of human neuraminidase (2018)
neuraminidase 2/Sialidase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3200] [GtoPdb: 3258] [UniProtKB: Q9Y3R4]
ChEMBL	Competitive inhibition of N-terminal MBP-fused human NEU2 expressed in Escherichia coli using 4MU-NANA as substrate preincubated with substrate for 15 mins and measured every min for 30 mins by Lineweaver-Burk plot analysis	B	5.89	pKi	1300	nM	Ki	J Med Chem (2018) 61: 11261-11279 [PMID:30457869]
GtoPdb	-	-	5.89	pKi	1300	nM	Ki	J Med Chem (2018) 61: 11261-11279 [PMID:30457869]
ChEMBL	Inhibition of N-terminal MBP-fused human NEU2 expressed in Escherichia coli using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition and measured after 30 mins by fluorescence assay	B	5.68	pIC50	2100	nM	IC50	J Med Chem (2018) 61: 11261-11279 [PMID:30457869]
ChEMBL	Inhibition of N-terminal MBP tagged human neuraminidase-2 expressed in Escherichia coli pre-incubated for 15 mins before 4MU-NANA substrate addition and measured after 30 mins by fluorescence based assay	B	5.68	pIC50	2100	nM	IC50	WO-2018213933-A1. Methods of preventing or treating atherosclerosis with inhibitors of specific isoenzymes of human neuraminidase (2018)
GtoPdb	-	-	5.68	pIC50	2100	nM	IC50	J Med Chem (2018) 61: 11261-11279 [PMID:30457869]
Sialidase 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4174] [UniProtKB: Q8WWR8]
ChEMBL	Inhibition of MBP-fused human NEU4 expressed in Escherichia coli using 4MU-NANA as substrate preincubated for 15 mins followed by substrate addition and measured after 30 mins by fluorescence assay	B	4.33	pIC50	47000	nM	IC50	J Med Chem (2018) 61: 11261-11279 [PMID:30457869]
ChEMBL	Inhibition of N-terminal GST tagged human neuraminidase-4 expressed in Escherichia coli pre-incubated for 15 mins before 4MU-NANA substrate addition and measured after 30 mins by fluorescence based assay	B	4.33	pIC50	47000	nM	IC50	WO-2018213933-A1. Methods of preventing or treating atherosclerosis with inhibitors of specific isoenzymes of human neuraminidase (2018)

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]