ChEMBL ligand: CHEMBL256147 (L-NMMA, Ng-methylarginine, Ng-monomethyl-l-arginine, .omega.-n-methylarginine, Omega-n-methylarginine, Omega-n-methylated arginine, Targinine, Tilarginina, Tilarginine)
Neuronal NOS/Nitric-oxide synthase, brain in Human [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475] Neuronal NOS/Nitric-oxide synthase, brain in Mouse [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4] Neuronal NOS/Nitric-oxide synthase, brain in Rat [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476] There should be some charts here, you may need to enable JavaScript!
Endothelial NOS/Nitric-oxide synthase, endothelial in Human [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474] Nitric-oxide synthase, endothelial in Bovine [ChEMBL: CHEMBL4802] [UniProtKB: P29473] There should be some charts here, you may need to enable JavaScript!
Inducible NOS/Nitric oxide synthase, inducible in Human [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228] Inducible NOS/Nitric oxide synthase, inducible in Mouse [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477] Inducible NOS/Nitric oxide synthase, inducible in Rat [ChEMBL: CHEMBL3051] [GtoPdb: 1250] [UniProtKB: Q06518] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Neuronal NOS/Nitric-oxide synthase, brain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3568] [GtoPdb: 1251] [UniProtKB: P29475]
ChEMBL	Inhibitory constant for the inhibition of human Neuronal nitric oxide synthase	B	6.08	pKi	840	nM	Ki	Bioorg Med Chem Lett (1997) 7: 1763-1768
ChEMBL	Inhibitory activity against human neuronal nitric oxide synthase (nNOS) isoenzyme.	B	6.08	pKi	840	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
GtoPdb	-	-	6.08	pKi	840	nM	Ki	Bioorganic and Medicinal Chemistry Letters (1997) 7: 1763-1768
ChEMBL	Inhibition of cloned (from RNA) human Neuronal nitric oxide synthase	B	5	pIC50	10000	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibition of human neuronal Nitric Oxide Synthase	B	5.31	pIC50	4900	nM	IC50	Bioorg Med Chem Lett (2000) 10: 597-600 [PMID:10741561]
ChEMBL	Inhibitory activity against neuronal nitric oxide synthase	B	5.57	pIC50	2700	nM	IC50	Bioorg Med Chem Lett (2000) 10: 1975-1978 [PMID:10987430]
ChEMBL	Inhibition of human nNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method	B	6.02	pIC50	950	nM	IC50	J Med Chem (2012) 55: 2882-2893 [PMID:22335555]
ChEMBL	Inhibition of human recombinant nNOS assessed as conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins measured after 45 mins by liquid scintillation counting	B	6.02	pIC50	950	nM	IC50	ACS Med Chem Lett (2012) 3: 227-231 [PMID:24900459]
ChEMBL	Concentration required to inhibit neuronal nitric oxide synthase	B	6.05	pIC50	890	nM	IC50	Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
ChEMBL	Inhibitory concentration against recombinant human (neuronal nitric oxide synthase) n-NOS	B	6.05	pIC50	890	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL	Inhibitory activity against human neuronal nitric oxide synthase	B	7.05	pIC50	90	nM	IC50	J Med Chem (2003) 46: 913-916 [PMID:12620067]
Neuronal NOS/Nitric-oxide synthase, brain in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4719] [GtoPdb: 1251] [UniProtKB: Q9Z0J4]
ChEMBL	Inhibition of neuronal nitric oxide synthase (nNOS) in mice	B	5.37	pIC50	4300	nM	IC50	Bioorg Med Chem Lett (2004) 14: 313-316 [PMID:14698148]
Neuronal NOS/Nitric-oxide synthase, brain in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3048] [GtoPdb: 1251] [UniProtKB: P29476]
ChEMBL	Compound was tested for binding affinity against neuronal nitric oxide synthase(nNOS)	B	5	pKi	10000	nM	Ki	J Med Chem (1997) 40: 3869-3870 [PMID:9397167]
ChEMBL	Inhibition of rat nNOS transfected in HEK293 cells assessed as inhibition constant in presence of NADPH by spectrophotometric analysis	B	6.3	pKi	500	nM	Ki	Bioorg Med Chem (2022) 72: 116970-116970 [PMID:36063653]
ChEMBL	Inhibition of rat nNOS	B	6.7	pKi	200	nM	Ki	J Med Chem (2013) 56: 3121-3147 [PMID:23458846]
ChEMBL	Compound was tested in vitro for inhibitory activity against nNOS (constitutive isoform found in neurons)	B	5	pIC50	10000	nM	IC50	Bioorg Med Chem Lett (1996) 6: 999-1002
ChEMBL	Inhibitory activity against isomeric form of Neuronal nitric oxide synthase measured by citrulline assay	B	5.12	pIC50	7500	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL	Ability to inhibit the conversion of [3H]-L-Arg to [3H]L-citrulline catalyzed by n-NOS from rat cerebellum	F	7	pIC50	100	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1027-1030 [PMID:11327581]
ChEMBL	Inhibition of conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by neuronal NOS (n NOS) from rat cerebellum	F	7	pIC50	100	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1023-1026 [PMID:11327580]
Endothelial NOS/Nitric-oxide synthase, endothelial in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4803] [GtoPdb: 1249] [UniProtKB: P29474]
ChEMBL	Inhibitory constant for the inhibition of human Endothelial nitric oxide synthase	B	6.4	pKi	400	nM	Ki	Bioorg Med Chem Lett (1997) 7: 1763-1768
ChEMBL	Inhibitory activity against human endothelial nitric oxide synthase (eNOS) isoenzyme.	B	6.4	pKi	400	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
GtoPdb	-	-	6.4	pKi	400	nM	Ki	Bioorganic and Medicinal Chemistry Letters (1997) 7: 1763-1768
ChEMBL	Inhibitory activity against endothelial nitric oxide synthase (eNOS)	B	5.06	pIC50	8700	nM	IC50	Bioorg Med Chem Lett (2000) 10: 1975-1978 [PMID:10987430]
ChEMBL	The compound was evaluated for the inhibitory potencies using human Endothelial nitric oxide synthase	B	5.22	pIC50	6000	nM	IC50	J Med Chem (1996) 39: 5110-5118 [PMID:8978842]
ChEMBL	Inhibition of cloned (from RNA) human endothelial constitutive Endothelial nitric oxide synthase (heNOS)	B	5.23	pIC50	5900	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibition of human endothelial Nitric Oxide Synthase	B	5.46	pIC50	3500	nM	IC50	Bioorg Med Chem Lett (2000) 10: 597-600 [PMID:10741561]
ChEMBL	Inhibition of human eNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method	B	6.19	pIC50	650	nM	IC50	J Med Chem (2012) 55: 2882-2893 [PMID:22335555]
ChEMBL	Inhibition of human recombinant eNOS assessed as conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins measured after 45 mins by liquid scintillation counting	B	6.19	pIC50	650	nM	IC50	ACS Med Chem Lett (2012) 3: 227-231 [PMID:24900459]
ChEMBL	Inhibitory concentration against recombinant human Endothelial nitric oxide synthase	B	6.27	pIC50	540	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL	In vitro inhibition of endothelial nitric oxide synthase.	B	6.27	pIC50	540	nM	IC50	Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
ChEMBL	Inhibitory activity against human endothelial nitric oxide synthase	B	6.48	pIC50	330	nM	IC50	J Med Chem (2003) 46: 913-916 [PMID:12620067]
ChEMBL	Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by e-NOS from HUVEC cells	F	6.52	pIC50	300	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1027-1030 [PMID:11327581]
ChEMBL	Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells	F	6.52	pIC50	300	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1023-1026 [PMID:11327580]
Nitric-oxide synthase, endothelial in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4802] [UniProtKB: P29473]
ChEMBL	Compound was tested for binding affinity against Endothelial nitric oxide synthase	B	5.23	pKi	5900	nM	Ki	J Med Chem (1997) 40: 3869-3870 [PMID:9397167]
ChEMBL	Inhibitory activity of conversion of radiolabeled arginine to citrulline by isomeric form of nitric oxide synthase Endothelial nitric oxide synthase from cultured bovine aortic endothelial cells	B	5.15	pIC50	7000	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
Inducible NOS/Nitric oxide synthase, inducible in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4481] [GtoPdb: 1250] [UniProtKB: P35228]
ChEMBL	Inhibitory constant for the inhibition of human Inducible nitric oxide synthase	B	6.07	pKi	860	nM	Ki	Bioorg Med Chem Lett (1997) 7: 1763-1768
ChEMBL	Inhibitory activity against human inducible nitric oxide synthase (iNOS) isoenzyme.	B	6.07	pKi	860	nM	Ki	J Med Chem (1998) 41: 2858-2871 [PMID:9667974]
GtoPdb	-	-	6.07	pKi	860	nM	Ki	Bioorganic and Medicinal Chemistry Letters (1997) 7: 1763-1768
ChEMBL	The compound was evaluated for the inhibitory potencies using human Inducible nitric oxide synthase (hiNOS)	B	4.57	pIC50	27000	nM	IC50	J Med Chem (1996) 39: 5110-5118 [PMID:8978842]
ChEMBL	Inhibition of cloned (from RNA) human inducible nitric oxide synthase (hiNOS)	B	4.85	pIC50	14000	nM	IC50	J Med Chem (1998) 41: 3675-3683 [PMID:9733492]
ChEMBL	Inhibitory activity against inducible nitric oxide synthase (iNOS)	B	5	pIC50	10000	nM	IC50	Bioorg Med Chem Lett (2000) 10: 1975-1978 [PMID:10987430]
ChEMBL	Inhibition of iNOS (unknown origin)	B	5.18	pIC50	6600	nM	IC50	Eur J Med Chem (2023) 248: 115112-115112 [PMID:36641860]
ChEMBL	Inhibition of human inducible nitric oxide synthase	B	5.18	pIC50	6600	nM	IC50	Bioorg Med Chem Lett (2000) 10: 597-600 [PMID:10741561]
ChEMBL	Inhibition of iNOS	B	5.47	pIC50	3400	nM	IC50	J Med Chem (2008) 51: 4150-4169 [PMID:18588282]
ChEMBL	Inhibition of human recombinant iNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method	B	5.74	pIC50	1800	nM	IC50	J Med Chem (2012) 55: 2882-2893 [PMID:22335555]
ChEMBL	Inhibitory concentration against human Inducible nitric oxide synthase	B	6	pIC50	1000	nM	IC50	Bioorg Med Chem Lett (2003) 13: 1981-1984 [PMID:12781178]
ChEMBL	In vitro inhibition of inducible nitric oxide synthase.	B	6	pIC50	1000	nM	IC50	Bioorg Med Chem Lett (2002) 12: 2561-2564 [PMID:12182860]
ChEMBL	Inhibitory activity of compound against human inducible nitric oxide synthase	B	6.47	pIC50	340	nM	IC50	J Med Chem (2003) 46: 913-916 [PMID:12620067]
ChEMBL	Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by i-NOS from human DLD-1 cells	F	6.52	pIC50	300	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1027-1030 [PMID:11327581]
ChEMBL	Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells	F	6.52	pIC50	300	nM	IC50	Bioorg Med Chem Lett (2001) 11: 1023-1026 [PMID:11327580]
ChEMBL	Inhibition of human recombinant iNOS assessed as conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins measured after 45 mins by liquid scintillation counting	B	8.74	pIC50	1.8	nM	IC50	ACS Med Chem Lett (2012) 3: 227-231 [PMID:24900459]
Inducible NOS/Nitric oxide synthase, inducible in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3464] [GtoPdb: 1250] [UniProtKB: P29477]
ChEMBL	Compound was tested for binding affinity against recombinant inducible nitric oxide synthase (iNOS) from mouse	B	4.85	pKi	14000	nM	Ki	J Med Chem (1997) 40: 3869-3870 [PMID:9397167]
ChEMBL	Compound was tested in vitro for inhibitory activity against inducible nitric oxide synthase	B	4.6	pIC50	25000	nM	IC50	Bioorg Med Chem Lett (1996) 6: 999-1002
ChEMBL	Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells	B	4.6	pIC50	25000	nM	IC50	J Med Chem (1998) 41: 2636-2642 [PMID:9651169]
ChEMBL	Inhibition of iNOS-mediated nitric oxide production in LPS-stimulated mouse RAW 264.7 cells pretreated 15 mins before LPS challenge measured after 20 hrs relative to control	B	4.66	pIC50	22100	nM	IC50	Bioorg Med Chem (2011) 19: 6182-6195 [PMID:21978950]
ChEMBL	Inhibition of LPS-induced iNOS activity in mouse RAW 264.7 cells assessed as inhibition of NO production pretreated with compound for 30 mins before LPS challenge after 24 hrs by Griess reagent method	B	4.71	pIC50	19700	nM	IC50	Bioorg Med Chem (2012) 20: 7030-7039 [PMID:23142320]
ChEMBL	Inhibition of inducible nitric oxide synthase (iNOS) in mice	B	4.72	pIC50	19000	nM	IC50	Bioorg Med Chem Lett (2004) 14: 313-316 [PMID:14698148]
ChEMBL	Inhibition of iNOS in ddY mouse peritoneal exudate cells after 20 hrs	B	4.89	pIC50	13000	nM	IC50	J Nat Prod (2004) 67: 1119-1124 [PMID:15270564]
ChEMBL	Tested for inhibition of mouse inducible nitric oxide synthase	B	5.08	pIC50	8300	nM	IC50	J Med Chem (1994) 37: 3886-3888 [PMID:7525961]
ChEMBL	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced iNOS activity incubated for 30 mins prior to LPS challenge measured after 24 hrs by Griess assay	B	5.64	pIC50	2300	nM	IC50	Bioorg Med Chem (2015) 23: 3248-3259 [PMID:25975638]
Inducible NOS/Nitric oxide synthase, inducible in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3051] [GtoPdb: 1250] [UniProtKB: Q06518]
ChEMBL	Tested for inhibition of rat brain inducible nitric oxide synthase	B	4.74	pIC50	18000	nM	IC50	J Med Chem (1994) 37: 3886-3888 [PMID:7525961]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]