ChEMBL ligand: CHEMBL532155 (MMV019313, TCMDC-123889)
Calcium-dependent protein kinase 1 in Plasmodium falciparum [ChEMBL: CHEMBL1908387] [UniProtKB: P62344] There should be some charts here, you may need to enable JavaScript!
Calcium-dependent protein kinase 4 in Plasmodium falciparum 3D7 [ChEMBL: CHEMBL2169725] [UniProtKB: Q8IBS5] There should be some charts here, you may need to enable JavaScript!
Cdk-related protein kinase 6 in Plasmodium falciparum [ChEMBL: CHEMBL3301558] [UniProtKB: O77239] There should be some charts here, you may need to enable JavaScript!
Glucose transporter in Leishmania mexicana [ChEMBL: CHEMBL3431938] [UniProtKB: O61059] Glucose transporter 1/Glucose transporter in Human [ChEMBL: CHEMBL2535] [GtoPdb: 875] [UniProtKB: P11166] There should be some charts here, you may need to enable JavaScript!
Hexose transporter 1 in Plasmodium falciparum [ChEMBL: CHEMBL4697] [UniProtKB: O97467] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum lysyl-tRNA synthetase/Lysine--tRNA ligase in Plasmodium falciparum 3D7 [ChEMBL: CHEMBL3301561] [GtoPdb: 3059] [UniProtKB: Q8IDJ8] There should be some charts here, you may need to enable JavaScript!
Mitogen-activated protein kinase in Plasmodium falciparum [ChEMBL: CHEMBL3301559] [UniProtKB: Q25917] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum prolyl-tRNA synthetase/Proline--tRNA ligase in Plasmodium falciparum 3D7 [ChEMBL: CHEMBL3301560] [GtoPdb: 3056] [UniProtKB: Q8I5R7] There should be some charts here, you may need to enable JavaScript!
Putative uncharacterized protein pk7 in Plasmodium falciparum [ChEMBL: CHEMBL6169] [UniProtKB: O96214] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum bifunctional farnesyl/geranylgeranyl diphosphate synthase in Plasmodium falciparum W2 [GtoPdb: 3173] Plasmodium falciparum bifunctional farnesyl/geranylgeranyl diphosphate synthase in Plasmodium falciparum Dd2 [GtoPdb: 3173] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Calcium-dependent protein kinase 1 in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1908387] [UniProtKB: P62344]
ChEMBL	MMV: CDPK1 Protein kinase inhibition assay using recombinant PfCDPK1, syntide 2 peptide substrate, ATP and KinaseGlo to measure % inhibition at 1 uM MMV box compound.	F	6	pIC50	>1000	nM	IC50	MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases.
Calcium-dependent protein kinase 4 in Plasmodium falciparum 3D7 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2169725] [UniProtKB: Q8IBS5]
ChEMBL	MMV: CDPK4 Protein kinase inhibition assay using recombinant PfCDPK4, syntide2 peptide substrate, ATP and KinaseGlo to measure % inhibition at 1 uM MMV box compound.	F	6	pIC50	>1000	nM	IC50	MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases.
Cdk-related protein kinase 6 in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3301558] [UniProtKB: O77239]
ChEMBL	MMV: PK6 Protein kinase inhibition assay using recombinant PfPK6, Myelin Basic Protein (MBP) substrate, ATP and KinaseGlo to measure % inhibition at 1 uM MMV box compound. Final concentrations were 1.5 uM ATP, 5 mM MnCl2, and 15 ug/ml PK6 in a buffer of 100 mM Tris-HCl (pH 7.5). 50 ug/mL MBP was provided as the substrate; 10 uM staurosporine was used as a control inhibitor. Incubation time was 3 hours and 40 minutes.The catalytic activity of each kinase was considered proportional to ATP consumed, as determined from measurements of residual [ATP] with the luciferase-based reagent Kinase-Glo (Promega) following incubation. Luminescence (proportional to residual [ATP]) was measured on the plate readers FLx800 (BioTek Instruments, Winooski, VT, USA) and MicroBeta2.	F	6	pIC50	>1000	nM	IC50	MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases.
Glucose transporter in Leishmania mexicana (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3431938] [UniProtKB: O61059]
ChEMBL	ST_JUDE_LEISH: Cytotoxicity against transgenic Leishmania Mexicana promastigotes LmGT2 that are glucose transport deficient and complemented with the L. Mexicana glucose transporter 2. Activity is measured by by DNA content using SYBR green in vitro	F	4.92	pIC50	>12000	nM	IC50	St. Jude Leishmania screening dataset.
Glucose transporter 1/Glucose transporter in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2535] [GtoPdb: 875] [UniProtKB: P11166]
ChEMBL	ST_JUDE_LEISH: Cytotoxicity against transgenic Leishmania Mexicana promastigotes LmGLUT1 that are glucose transport deficient and complemented with the human glucose transporter GLUT1. Activity is measured by DNA content using SYBR green in vitro	F	4.92	pIC50	>12000	nM	IC50	St. Jude Leishmania screening dataset.
Hexose transporter 1 in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4697] [UniProtKB: O97467]
ChEMBL	ST_JUDE_LEISH: Cytotoxicity against transgenic Leishmania Mexicana promastigotes LmPfHT that are glucose transport deficient and complemented with the Plasmodium falciparum hexose transporter. Activity is measured by by DNA content using SYBR green in vitro	F	4.92	pIC50	>12000	nM	IC50	St. Jude Leishmania screening dataset.
Plasmodium falciparum lysyl-tRNA synthetase/Lysine--tRNA ligase in Plasmodium falciparum 3D7 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3301561] [GtoPdb: 3059] [UniProtKB: Q8IDJ8]
ChEMBL	MMV: KRS recombinant Plasmodium falciparum Lysyl-tRNA-synthetase, assay with yeast tRNA, ATP, and lysine and 3 uM MMV malaria box compound, assay read out with kinase glo as % inhibition.	F	5.6	pIC50	>2500	nM	IC50	MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases.
Mitogen-activated protein kinase in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3301559] [UniProtKB: Q25917]
ChEMBL	MMV: MAP2K Protein kinase inhibition assay using recombinant PfMAP2K, protein substrate, ATP and KinaseGlo to measure % inhibition at 1 uM MMV box compound. Final concentrations were 1 uM ATP, 0.5 mM DTT, 1 mM MgCl2, 0.5 mg/mL BSA, and 10 ug/ml MAP2 in a buffer of 50 mM HEPES (pH 7.0). 0.5 mg/mL histone III-S served as the substrate (13); 100 uM AMP-PNP, an ATP analog, was used as a control inhibitor. Incubation time was 4 hours.	F	6	pIC50	>1000	nM	IC50	MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases.
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL	MMV: IC50 determined against P. falciparum Dd2-luc strain using a Malaria Sybr Green I Fluorescence assay. Starting conditions were 1% trophozoite synchronised parasites at 2% haematocrit. Fluorescence detection completed after 48hr incubation at 37 degrees C under 1% O2, 3% CO2 and balance N2.	F	6.44	pIC50	366.4	nM	IC50	MMV box screening in the Dd2 P. falciparum strain
ChEMBL	Antimalarial activity against Plasmodium falciparum infected in human RBC assessed as reduction in parasite growth measured after 72 hrs by flow cytometry analysis	F	7.05	pIC50	90	nM	IC50	Bioorg Med Chem Lett (2021) 41: 127978-127978 [PMID:33766764]
ChEMBL	MMV: Inhibition of Plasmodium falciparum 3D7 (EC50).	F	6.17	pEC50	669	nM	EC50	Malaria Box
ChEMBL	MMV: Inhibition of Plasmodium falciparum K1 (EC50).	F	6.24	pEC50	576	nM	EC50	Malaria Box
Plasmodium falciparum prolyl-tRNA synthetase/Proline--tRNA ligase in Plasmodium falciparum 3D7 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3301560] [GtoPdb: 3056] [UniProtKB: Q8I5R7]
ChEMBL	MMV: ProRS recombinant Plasmodium falciparum Prolyl-tRNA-synthetase, assay with yeast tRNA, ATP, and proline and 3 uM MMV malaria box compound, assay read out with kinase glo as % inhibition.	F	5.6	pIC50	>2500	nM	IC50	MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases.
Putative uncharacterized protein pk7 in Plasmodium falciparum (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6169] [UniProtKB: O96214]
ChEMBL	MMV: PK7 Protein kinase inhibition assay using recombinant PfPK7, ATP and KinaseGlo to measure % inhibition at 1 uM MMV box compound. Final concentrations were 1 uM ATP, 2 mM DTT, 20 mM MgCl2, 2 mM MnCl2, 0.01% BSA, and 6 ug/ml PK7, in a buffer of 20 mM Tris-HCl (pH 7.5). The enzyme itself was the only substrate present (since autophosphorylation occurs); 100 uM 1NA-PP1 was used as a control inhibitor. Incubation time was 3 hours.	F	6	pIC50	>1000	nM	IC50	MMV Box screening against Plasmodium falciparum protein kinases and aminoacyl tRNA synthetases.
Plasmodium falciparum bifunctional farnesyl/geranylgeranyl diphosphate synthase in Plasmodium falciparum W2 [GtoPdb: 3173]
GtoPdb	-	-	6.57	pEC50	270	nM	EC50	Cell Chem Biol (2018) 25: 185-193.e5 [PMID:29276048]
Plasmodium falciparum bifunctional farnesyl/geranylgeranyl diphosphate synthase in Plasmodium falciparum Dd2 [GtoPdb: 3173]
GtoPdb	-	-	6.74	pEC50	180	nM	EC50	Cell Chem Biol (2018) 25: 185-193.e5 [PMID:29276048]

ChEMBL data shown on this page come from version 32:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]