ChEMBL ligand: CHEMBL3764617
sphingosine kinase 1/Sphingosine kinase 1 in Human [ChEMBL: CHEMBL4394] [GtoPdb: 2204] [UniProtKB: Q9NYA1] There should be some charts here, you may need to enable JavaScript!
sphingosine kinase 2/Sphingosine kinase 2 in Human [ChEMBL: CHEMBL3023] [GtoPdb: 2205] [UniProtKB: Q9NRA0] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
sphingosine kinase 1/Sphingosine kinase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4394] [GtoPdb: 2204] [UniProtKB: Q9NYA1]
ChEMBL	Inhibition of human SK1 using D-erythro sphingosine as substrate and gamma[33P]ATP by Lineweaver-Burk plot analysis	B	4.57	pKi	27000	nM	Ki	J Med Chem (2016) 59: 965-984 [PMID:26780304]
ChEMBL	Inhibition of recombinant human C-terminal TEV cleavage site-fused-His6-tagged SphK1 expressed in baculovirus infected sf9 insect cells using d-erythro-sphingosine as substrate in presence of [gamma32P]-ATP and ATP by radioactivity-based assay	B	4.57	pKi	27000	nM	Ki	J Med Chem (2020) 63: 1178-1198 [PMID:31895563]
ChEMBL	Inhibition of recombinant human SphK1 expressed in baculovirus infected Sf9 cells assessed as decrease in [33P]SIP production using D-erythro-sphingosine as substrate in presence of [gamma33P]-ATP by scintillation counting method	B	4.57	pKi	27000	nM	Ki	Bioorg Med Chem (2021) 30: 115941-115941 [PMID:33385956]
ChEMBL	Inhibition of recombinant human SphK1 expressed in baculovirus infected Sf9 cells using d-erythro-sphingosine and [gamma-32P]ATP as substrate by liquid scintillation counting method	B	4.57	pKi	27000	nM	Ki	Eur J Med Chem (2021) 213: 113164-113164 [PMID:33454547]
GtoPdb	In vitro inhibition of substrate phosphorylation.	-	4.57	pIC50	27000	nM	IC50	Oncotarget (2015) 6: 7065-83 [PMID:25788259]
sphingosine kinase 2/Sphingosine kinase 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3023] [GtoPdb: 2205] [UniProtKB: Q9NRA0]
GtoPdb	In vitro inhibition of substrate phosphorylation.	-	5.16	pKi	6900	nM	Ki	Oncotarget (2015) 6: 7065-83 [PMID:25788259]
ChEMBL	Inhibition of human SK2 using D-erythro sphingosine as substrate and gamma[33P]ATP by Lineweaver-Burk plot analysis	B	5.16	pKi	6900	nM	Ki	J Med Chem (2016) 59: 965-984 [PMID:26780304]
ChEMBL	Inhibition of recombinant human His6/HA-fusion-tagged SphK2 expressed in baculovirus infected sf9 insect cells using d-erythro-sphingosine as substrate in presence of [gamma32P]-ATP and ATP by radioactivity-based assay	B	5.16	pKi	6900	nM	Ki	J Med Chem (2020) 63: 1178-1198 [PMID:31895563]
ChEMBL	Inhibition of recombinant human SphK2 assessed as decrease in [33P]SIP production using sphingosine as substrate in presence of [gamma33P]-ATP by scintillation counting method	B	5.16	pKi	6900	nM	Ki	Bioorg Med Chem (2021) 30: 115941-115941 [PMID:33385956]
ChEMBL	Inhibition of recombinant human SphK2 expressed in baculovirus infected Sf9 cells using d-erythro-sphingosine and [gamma-32P]ATP as substrate by liquid scintillation counting method	B	5.16	pKi	6900	nM	Ki	Eur J Med Chem (2021) 213: 113164-113164 [PMID:33454547]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]