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Kir2.2

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Target id: 431

Nomenclature: Kir2.2

Family: Inwardly rectifying potassium channels (KIR)

Contents:

Gene and Protein Information Click here for help
Species TM P Loops AA Chromosomal Location Gene Symbol Gene Name Reference
Human 2 1 433 17p11.2 KCNJ12 potassium inwardly rectifying channel subfamily J member 12 4,14
Mouse 2 1 427 11 37.96 cM Kcnj12 potassium inwardly-rectifying channel, subfamily J, member 12 11-12
Rat 2 1 427 10q22 Kcnj12 potassium inwardly-rectifying channel, subfamily J, member 12 6
Previous and Unofficial Names Click here for help
IRK2 | MB-IRK2 | KCNJN1 | ATP-sensitive inward rectifier potassium channel 12 | RB-IRK2 | potassium channel, inwardly rectifying subfamily J, member 12 | potassium inwardly-rectifying channel | potassium inwardly rectifying channel subfamily J member 12
Database Links Click here for help
Alphafold Q14500 (Hs), P52187 (Mm), P52188 (Rn)
CATH/Gene3D 2.60.40.1400
DrugBank Target Q14500 (Hs)
Ensembl Gene ENSG00000184185 (Hs), ENSMUSG00000042529 (Mm), ENSRNOG00000002303 (Rn)
Entrez Gene 3768 (Hs), 16515 (Mm), 117052 (Rn)
Human Protein Atlas ENSG00000184185 (Hs)
KEGG Gene hsa:3768 (Hs), mmu:16515 (Mm), rno:117052 (Rn)
OMIM 602323 (Hs)
Pharos Q14500 (Hs)
RefSeq Nucleotide NM_021012 (Hs), NM_010603 (Mm), NM_053981 (Rn)
RefSeq Protein NP_066292 (Hs), NP_034733 (Mm), NP_446433 (Rn)
UniProtKB Q14500 (Hs), P52187 (Mm), P52188 (Rn)
Wikipedia KCNJ12 (Hs)
Associated Proteins Click here for help
Heteromeric Pore-forming Subunits
Name References
Kir2.1 9,16
Auxiliary Subunits
Name References
Not determined
Other Associated Proteins
Name References
chapsyn-110 7
veli-1 7-8
veli-3 7-8
α1-, β1-, β2-syntrophin 7
α-dystrobrevin-2 7
α-dystrobrevin-1 7
Dp71 7
dystrophin 7
actin-binding LIM protein 7
Dlg3 7
Dlg2 7
SAP-97 7-8
PSD-95 7
SAP102 7
CASK 7-8
Functional Characteristics Click here for help
IK1 in heart, ‘strong’ inward–rectifier current
Ion Selectivity and Conductance Click here for help
Species:  Mouse
Rank order:  K+ [34.2 pS]
References:  11

Download all structure-activity data for this target as a CSV file go icon to follow link

Inhibitors
Key to terms and symbols Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
Intracellular Mg2+ Click here for species-specific activity table Ligand is endogenous in the given species Hs - 5.0 pIC50 - 40.0 15
⤷ pIC50 5.0 (IC50 1.1x10-5 M) [15]
Holding voltage: 40.0 mV
Gating inhibitors Click here for help
Key to terms and symbols Click column headers to sort
Ligand Sp. Action Value Parameter Concentration range (M) Holding voltage (mV) Reference
Ba2+ Click here for species-specific activity table Mm Antagonist - - 5x10-5 -150.0 – -50.0 11
⤷ Conc range: 5x10-5 M [11]
Holding voltage: -150.0 – -50.0 mV
Cs+ Click here for species-specific activity table Mm Antagonist - - 5x10-6 - 5x10-5 -150.0 – -50.0 11
⤷ Conc range: 5x10-6 - 5x10-5 M [11]
Holding voltage: -150.0 – -50.0 mV
Ba2+ Click here for species-specific activity table Rn Antagonist 5.2 pKi - -80.0 13
⤷ pKi 5.2 [13]
Holding voltage: -80.0 mV
View species-specific gating inhibitor tables
Gating Inhibitor Comments
Endogenous inhibitors are intracellular Mg2+ and polyamines (spermine4+, spermidine3+, putrescine2+).
Tissue Distribution Click here for help
Aortic endothelial cells.
Species:  Human
Technique:  Western Blot
References:  2
Cerebellum > motor trigeminal nucleus > olfactory bulb, piriform cortex, cerebral cortex, hippocampus, dentate gyrus, pontine nucleus.
Species:  Mouse
Technique:  In situ hybridisation
References:  3
Heart, forebrain, cerebellum, skeletal muscle.
Species:  Mouse
Technique:  Northern Blot
References:  11
Medial habenula, spinal medulla > cortex, hippocampus, basal ganglia (caudate putamen, nucleus accumbens, globus pallidus), thalamus, substantia nigra, cerebellum > hypothalamus.
Species:  Rat
Technique:  Immunohistochemistry
References:  10
Thalamus > cerebellum (granule cell layer), brainstem (inf. olivary, motor trigeminal, facial, cochlear and hypoglossal nuclei) > basal ganglia (habenula, vertical limb of the diagonal band) > superior colliculus.
Species:  Rat
Technique:  In situ hybridisation
References:  5
Functional Assays Click here for help
Patch clamp measurement of the K+ current in cardiac myocytes of Kir2.2 knockout mice using patch clamp.
Species:  Mouse
Tissue:  Heart.
Response measured:  Reduction of IK1 current.
References:  16
Physiological Functions Click here for help
Maintenance of the resting membrane potential and regulation of the excitability in neurons.
Species:  Mouse
Tissue:  Brain.
References:  1
Formation of the heart IK1 current, with Kir2.1.
Species:  Mouse
Tissue:  Heart.
References:  16

References

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1. Day M, Carr DB, Ulrich S, Ilijic E, Tkatch T, Surmeier DJ. (2005) Dendritic excitability of mouse frontal cortex pyramidal neurons is shaped by the interaction among HCN, Kir2, and Kleak channels. J Neurosci, 25 (38): 8776-87. [PMID:16177047]

2. Fang Y, Schram G, Romanenko VG, Shi C, Conti L, Vandenberg CA, Davies PF, Nattel S, Levitan I. (2005) Functional expression of Kir2.x in human aortic endothelial cells: the dominant role of Kir2.2. Am J Physiol, Cell Physiol, 289 (5): C1134-44. [PMID:15958527]

3. Horio Y, Morishige K, Takahashi N, Kurachi Y. (1996) Differential distribution of classical inwardly rectifying potassium channel mRNAs in the brain: comparison of IRK2 with IRK1 and IRK3. FEBS Lett, 379 (3): 239-43. [PMID:8603697]

4. Hugnot JP, Pedeutour F, Le Calvez C, Grosgeorge J, Passage E, Fontes M, Lazdunski M. (1997) The human inward rectifying K+ channel Kir 2.2 (KCNJ12) gene: gene structure, assignment to chromosome 17p11.1, and identification of a simple tandem repeat polymorphism. Genomics, 39 (1): 113-6. [PMID:9027495]

5. Karschin C, Dissmann E, Stühmer W, Karschin A. (1996) IRK(1-3) and GIRK(1-4) inwardly rectifying K+ channel mRNAs are differentially expressed in the adult rat brain. J Neurosci, 16 (11): 3559-70. [PMID:8642402]

6. Koyama H, Morishige K, Takahashi N, Zanelli JS, Fass DN, Kurachi Y. (1994) Molecular cloning, functional expression and localization of a novel inward rectifier potassium channel in the rat brain. FEBS Lett, 341 (2-3): 303-7. [PMID:8137958]

7. Leonoudakis D, Conti LR, Anderson S, Radeke CM, McGuire LM, Adams ME, Froehner SC, Yates 3rd JR, Vandenberg CA. (2004) Protein trafficking and anchoring complexes revealed by proteomic analysis of inward rectifier potassium channel (Kir2.x)-associated proteins. J Biol Chem, 279 (21): 22331-46. [PMID:15024025]

8. Leonoudakis D, Conti LR, Radeke CM, McGuire LM, Vandenberg CA. (2004) A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels. J Biol Chem, 279 (18): 19051-63. [PMID:14960569]

9. Preisig-Müller R, Schlichthörl G, Goerge T, Heinen S, Brüggemann A, Rajan S, Derst C, Veh RW, Daut J. (2002) Heteromerization of Kir2.x potassium channels contributes to the phenotype of Andersen's syndrome. Proc Natl Acad Sci USA, 99 (11): 7774-9. [PMID:12032359]

10. Prüss H, Derst C, Lommel R, Veh RW. (2005) Differential distribution of individual subunits of strongly inwardly rectifying potassium channels (Kir2 family) in rat brain. Brain Res Mol Brain Res, 139 (1): 63-79. [PMID:15936845]

11. Takahashi N, Morishige K, Jahangir A, Yamada M, Findlay I, Koyama H, Kurachi Y. (1994) Molecular cloning and functional expression of cDNA encoding a second class of inward rectifier potassium channels in the mouse brain. J Biol Chem, 269 (37): 23274-9. [PMID:8083233]

12. Takumi T, Tsuji L, Kondo C, Takahashi N, Morishige K, Copeland NG, Gilbert DJ, Jenkins NA, Kurachi Y. (1996) Assignment of the murine inward rectifier potassium channel Irk2 (Kir2.2) gene to the central region of mouse chromosome 11. Genomics, 37 (2): 270-2. [PMID:8921409]

13. Töpert C, Döring F, Wischmeyer E, Karschin C, Brockhaus J, Ballanyi K, Derst C, Karschin A. (1998) Kir2.4: a novel K+ inward rectifier channel associated with motoneurons of cranial nerve nuclei. J Neurosci, 18 (11): 4096-105. [PMID:9592090]

14. Wible BA, De Biasi M, Majumder K, Taglialatela M, Brown AM. (1995) Cloning and functional expression of an inwardly rectifying K+ channel from human atrium. Circ Res, 76 (3): 343-50. [PMID:7859381]

15. Yamashita T, Horio Y, Yamada M, Takahashi N, Kondo C, Kurachi Y. (1996) Competition between Mg2+ and spermine for a cloned IRK2 channel expressed in a human cell line. J Physiol (Lond.), 493 ( Pt 1): 143-56. [PMID:8735700]

16. Zaritsky JJ, Redell JB, Tempel BL, Schwarz TL. (2001) The consequences of disrupting cardiac inwardly rectifying K(+) current (I(K1)) as revealed by the targeted deletion of the murine Kir2.1 and Kir2.2 genes. J Physiol (Lond.), 533 (Pt 3): 697-710. [PMID:11410627]

Contributors

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