Gene and Protein Information
Species	TM	AA	Chromosomal Location	Gene Symbol	Gene Name	Reference
Plasmodium falciparum 3D7	-	832		eEF2	elongation factor 2
Gene and Protein Information Comments
Our eukaryotic translation elongation factor 2 summary page provides information about the homologous human target.

Previous and Unofficial Names

eEF2 | Pf3D7_14_v3:2,089,730..2,094,221(+) | PF14_0486

Database Links
Alphafold	Q8IKW5 (Pf3D7)
PlasmoDB	PF3D7_1451100 (Pf3D7)
UniProtKB	Q8IKW5 (Pf3D7)

Whole Organism Assay Data Linked to This Target
Key to terms and symbols		Click column headers to sort
Ligand Sp. Assay description Type Action Value Parameter Reference M5717 Pf3D7 Plasmodium falciparum 3D7 P. falciparum strain 3D7 (Pf3D7) was derived from isolate NF54 by limiting dilution. The complete genome of Pf3D7 has been sequenced (GenBank: LN999946.1). Pf3D7 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine. ✖ Parasite growth inhibition assay - - 9.0 pEC50 1 ⤷ pEC50 9.0 (EC50 1x10-9 M) [3H]-hypoxanthine incorporation assay [1] Lifecycle stages: Plasmodium asexual blood stage (erythrocytic merozoite, trophozoite, erythrocytic schizont) M5717 PfNF54 Plasmodium falciparum NF54 P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands. PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine. ✖ Parasite gamete formation assay - - 8.7 – 8.9 pEC50 1 ⤷ pEC50 8.9 (EC50 1.2x10-9 M) Female gamete formation [1] Lifecycle stages: Plasmodium mosquito host stage (gametocyte, gamete, zygote, ookinete, oocyst, sporozoite) pEC50 8.7 (EC50 1.8x10-9 M) Male gamete formation [1] Lifecycle stages: Plasmodium mosquito host stage (gametocyte, gamete, zygote, ookinete, oocyst, sporozoite) M5717 Pb Plasmodium berghei P. berghei (Pb) is a malaria parasite that infects mammals other than humans with the natural mammalian host being the thicket rat (G. surdaster) from Central Africa. Pb is used in rodent model studies of malaria. ✖ Parasite liver stage assay - - 8.8 pEC50 1 ⤷ pEC50 8.8 (EC50 1.65x10-9 M) P. berghei Luciferase liver stage assay [1] Lifecycle stages: Plasmodium liver stage (sporozoite, hepatic schizont, hepatic merozoite) M5717 PfNF54 Plasmodium falciparum NF54 P. falciparum strain NF54 (PfNF54) was derived from a patient who had never left the Netherlands. PfNF54 can be obtained from the European Malaria Reagent Repository or the Malaria Research and Reference Reagent Resource Center (MR4) and is sensitive to a panel of antimalarial compounds including chloroquine and pyrimethamine. ✖ Standard membrane feeding assay (SMFA) - - 8.0 – 8.7 pEC50 1 ⤷ pEC50 8.7 (EC50 1.8x10-9 M) Indirect SMFA [1] Lifecycle stages: Plasmodium mosquito host stage (gametocyte, gamete, zygote, ookinete, oocyst, sporozoite) pEC50 8.0 (EC50 1x10-8 M) Direct SMFA [1] Lifecycle stages: Plasmodium mosquito host stage (gametocyte, gamete, zygote, ookinete, oocyst, sporozoite)

Malaria Pharmacology Comments

Elongation factor 2 (eEF2) catalyzes the GTP-dependent translocation of the ribosome along messenger RNA, and is essential for protein synthesis in eukaryotes. Studies in S. cerevisiae have shown that this highly conserved protein is the target for the antifungal compound sordarin [3-4], while Plasmodium eEF2 (PfeEF2) has been identified as a novel target for antimalarial drug development [1] and a key target for interruption of malaria transmission [2].