ChEMBL ligand: CHEMBL848 (CC-5013, CDC-501, Lenalidomide, NSC-747972, Revlimid, SYP-1512)
bromodomain containing 4/Bromodomain-containing protein 4 in Human [ChEMBL: CHEMBL1163125] [GtoPdb: 1945] [UniProtKB: O60885] There should be some charts here, you may need to enable JavaScript!
cereblon/Cereblon/Ikaros in Human [ChEMBL: CHEMBL4296103] [GtoPdb: 3086] [UniProtKB: Q13422, Q96SW2] There should be some charts here, you may need to enable JavaScript!
Cereblon isoform 4 in Magnetospirillum gryphiswaldense [ChEMBL: CHEMBL3763007] [UniProtKB: A4TVL0] There should be some charts here, you may need to enable JavaScript!
cereblon/Protein cereblon in Human [ChEMBL: CHEMBL3763008] [GtoPdb: 3086] [UniProtKB: Q96SW2] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
bromodomain containing 4/Bromodomain-containing protein 4 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1163125] [GtoPdb: 1945] [UniProtKB: O60885]
ChEMBL	Inhibition of BRD4 bromodomain 2 (unknown origin) in HEK293 cells cotransfected with eGFP by flow cytometry based mCherry reporter gene analysis	B	5.28	pIC50	5190	nM	IC50	ACS Med Chem Lett (2020) 11: 1088-1089 [PMID:32550984]
cereblon/Cereblon/Ikaros in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL4296103] [GtoPdb: 3086] [UniProtKB: Q13422, Q96SW2]
GtoPdb	Measuring displacement of a Cy5-conjugated cereblon modulating compound from the binding pocket of CRBN by lenalidomide.	-	5.82	pIC50	1500	nM	IC50	J Med Chem (2018) 61: 535-542 [PMID:28425720]
ChEMBL	Induction of cereblon-mediated ikaros degradation in human DF15 cells expressing ePL-tagged ikaros after 4 hrs by luminometric analysis	B	7.17	pEC50	67	nM	EC50	J Med Chem (2018) 61: 535-542 [PMID:28425720]
Cereblon isoform 4 in Magnetospirillum gryphiswaldense (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3763007] [UniProtKB: A4TVL0]
ChEMBL	Inhibition of MANT-uracil binding to wild-type Magnetospirillum gryphiswaldense CRBN isoform 4 by FRET assay	B	5.51	pKi	3100	nM	Ki	J Med Chem (2019) 62: 6615-6629 [PMID:31251063]
cereblon/Protein cereblon in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3763008] [GtoPdb: 3086] [UniProtKB: Q96SW2]
ChEMBL	Binding affinity to human CRBN-thalidomide binding domain expressed in Escherichia coli by measuring baseline corrected normalized fluorescence by MST based assay	B	4.79	pKi	16300	nM	Ki	ACS Med Chem Lett (2021) 12: 74-81 [PMID:33488967]
ChEMBL	Binding affinity to CBRN (unknown origin) incubated for 60 mins by lenalidomide displacement assay	B	5.83	pKi	1490	nM	Ki	ACS Med Chem Lett (2020) 11: 1088-1089 [PMID:32550984]
ChEMBL	Binding affinity to human CRBN-thalidomide binding domain expressed in Escherichia coli by measuring baseline corrected normalized fluorescence by MST based assay	B	4.42	pIC50	37800	nM	IC50	ACS Med Chem Lett (2021) 12: 74-81 [PMID:33488967]
GtoPdb	Measuring displacement of a Cy5-conjugated cereblon modulating compound from the binding pocket of CRBN by lenalidomide.	-	5.82	pIC50	1500	nM	IC50	J Med Chem (2018) 61: 535-542 [PMID:28425720]
ChEMBL	Displacement of cy5-conjugated 2-((1E,3E,5Z)-5-(1-(6-((4-(2-((2-(2,6-Dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl)oxy)acetamido)butyl)amino)-6-oxohexyl)-3,3-dimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-1,3,3-trimethyl-3H-indol-1-ium from DDB1-fused human full length 6xHis-tagged CRBN expressed in Sf9 insect cells after 10 mins by TR-FRET assay	B	5.82	pIC50	1500	nM	IC50	J Med Chem (2018) 61: 535-542 [PMID:28425720]
ChEMBL	Displacement of fluorescent tracer from NanoLuc-tagged CRBN (unknown origin) by NanoBRET assay	B	6.41	pIC50	390	nM	IC50	RSC Med Chem (2023) 14: 501-506 [PMID:36970148]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]