adenosine 5'-monophosphate [Ligand Id: 2455] activity data from GtoPdb and ChEMBL

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ChEMBL ligand: CHEMBL752 (5'-adenylic acid, A 5MP, A-5MP, Adenosine 5'-monophosphoric acid, Adenosine Monophosphate, Adenosine phosphate, Fosfato de adenosina, NSC-20264, Phosphate d'adenosine, Vitamin b8)
  • 2`-deoxynucleoside 5`-phosphate N-hydrolase 1 in Human [ChEMBL: CHEMBL3351218] [UniProtKB: O43598]
  • 2`-deoxynucleoside 5`-phosphate N-hydrolase 1 in Rat [ChEMBL: CHEMBL3329079] [UniProtKB: O35820]
Created with Highcharts 10.3.3ValuesChart context menu2`-deoxynucleoside 5`-phosphate N-hydrolase 1pKd HumanpKi HumanpIC50 HumanpEC50 HumanpKd RatpKi RatpIC50 RatpEC50 Rat012345Highcharts.com
  • A1 receptor/Adenosine A1 receptor in Human [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542]
Created with Highcharts 10.3.3ValuesChart context menuAdenosine A1 receptorpKd HumanpKi HumanpIC50 HumanpEC50 Human01234567Highcharts.com
Created with Highcharts 10.3.3ValuesChart context menuAdenylate kinase 2pKd RatpKi RatpIC50 RatpEC50 Rat012345Highcharts.com
  • protein kinase AMP-activated non-catalytic subunit beta 2/protein kinase AMP-activated non-catalytic subunit gamma 1/protein kinase AMP-activated catalytic subunit alpha 2/protein kinase AMP-activated catalytic subunit alpha 1/protein kinase AMP-activated non-catalytic subunit gamma 3/protein kinase AMP-activated non-catalytic subunit gamma 2/protein kinase AMP-activated non-catalytic subunit beta 1/AMP-activated protein kinase, AMPK in Human [ChEMBL: CHEMBL2096907] [GtoPdb: 1544, 1545, 1542, 1541, 1547, 1546, 1543] [UniProtKB: O43741, P54619, P54646, Q13131, Q9UGI9, Q9UGJ0, Q9Y478]
Created with Highcharts 10.3.3ValuesChart context menuAMP-activated protein kinase, AMPKpKd HumanpKi HumanpIC50 HumanpEC50 Human0123456Highcharts.com
  • protein kinase AMP-activated non-catalytic subunit gamma 1/protein kinase AMP-activated catalytic subunit alpha 1/protein kinase AMP-activated non-catalytic subunit beta 1/AMP-activated protein kinase (AMPK) alpha-1/beta-1/gamma-1 in Human [ChEMBL: CHEMBL2111345] [GtoPdb: 1545, 1541, 1543] [UniProtKB: P54619, Q13131, Q9Y478]
Created with Highcharts 10.3.3ValuesChart context menuAMP-activated protein kinase (AMPK) alpha-1/beta-1/gamma-1pKd HumanpKi HumanpIC50 HumanpEC50 Human01234567Highcharts.com
  • protein kinase AMP-activated non-catalytic subunit gamma 1/protein kinase AMP-activated catalytic subunit alpha 2/protein kinase AMP-activated non-catalytic subunit beta 1/AMPK alpha2/beta1/gamma1 in Human [ChEMBL: CHEMBL3038455] [GtoPdb: 1545, 1542, 1543] [UniProtKB: P54619, P54646, Q9Y478]
Created with Highcharts 10.3.3ValuesChart context menuAMPK alpha2/beta1/gamma1pKd HumanpKi HumanpIC50 HumanpEC50 Human01234567Highcharts.com
Created with Highcharts 10.3.3ValuesChart context menuFructose-1,6-bisphosphatasepKd HumanpKi HumanpIC50 HumanpEC50 HumanpKd PigpKi PigpIC50 PigpEC50 Pig02468Highcharts.com
  • Human immunodeficiency virus type 1 protease in Human immunodeficiency virus 1 [ChEMBL: CHEMBL243] [UniProtKB: Q72874]
Created with Highcharts 10.3.3ValuesChart context menuHuman immunodeficiency virus type 1 proteasepKd Human immunodeficiency virus 1pKi Human immunodeficiency virus 1pIC50 Human immunodeficiency virus 1pEC50 Human immunodeficiency virus 1024681012Highcharts.com
  • Ketopantoate reductase in Escherichia coli [ChEMBL: CHEMBL4855] [UniProtKB: P0A9J4]
Created with Highcharts 10.3.3ValuesChart context menuKetopantoate reductasepKd Escherichia colipKi Escherichia colipIC50 Escherichia colipEC50 Escherichia coli0123Highcharts.com
  • L-lactate dehydrogenase A chain in Human [ChEMBL: CHEMBL4835] [UniProtKB: P00338]
Created with Highcharts 10.3.3ValuesChart context menuL-lactate dehydrogenase A chainpKd HumanpKi HumanpIC50 HumanpEC50 Human01234Highcharts.com
Created with Highcharts 10.3.3ValuesChart context menuP2Y purinoceptor 1pKd TurkeypKi TurkeypIC50 TurkeypEC50 Turkey0123456Highcharts.com
  • P2Y1 receptor/Purinergic receptor P2Y1 in Human [ChEMBL: CHEMBL4315] [GtoPdb: 323] [UniProtKB: P47900]
Created with Highcharts 10.3.3ValuesChart context menuPurinergic receptor P2Y1pKd HumanpKi HumanpIC50 HumanpEC50 Human01234567Highcharts.com
  • P2Y11 receptor/Purinergic receptor P2Y11 in Human [ChEMBL: CHEMBL4867] [GtoPdb: 327] [UniProtKB: Q96G91]
Created with Highcharts 10.3.3ValuesChart context menuPurinergic receptor P2Y11pKd HumanpKi HumanpIC50 HumanpEC50 Human012345Highcharts.com
  • P2Y2 receptor/Purinergic receptor P2Y2 in Human [ChEMBL: CHEMBL4398] [GtoPdb: 324] [UniProtKB: P41231]
Created with Highcharts 10.3.3ValuesChart context menuPurinergic receptor P2Y2pKd HumanpKi HumanpIC50 HumanpEC50 Human02468Highcharts.com
  • TRPM2/Transient receptor potential cation channel subfamily M member 2 in Human [ChEMBL: CHEMBL1250402] [GtoPdb: 494] [UniProtKB: O94759]
Created with Highcharts 10.3.3ValuesChart context menuTransient receptor potential cation channel subfamily M member 2pKd HumanpKi HumanpIC50 HumanpEC50 Human012345Highcharts.com
  • SRC proto-oncogene, non-receptor tyrosine kinase/Tyrosine-protein kinase SRC in Human [ChEMBL: CHEMBL267] [GtoPdb: 2206] [UniProtKB: P12931]
Created with Highcharts 10.3.3ValuesChart context menuTyrosine-protein kinase SRCpKd HumanpKi HumanpIC50 HumanpEC50 Human02468Highcharts.com
  • TRPM4 in Human [GtoPdb: 496] [UniProtKB: Q8TD43]
Created with Highcharts 10.3.3ValuesChart context menuTRPM4pKd HumanpKi HumanpIC50 HumanpEC50 Human012345Highcharts.com
  • AMP kinase in Human [GtoPdb: 1540]
Created with Highcharts 10.3.3ValuesChart context menuAMP kinasepKd HumanpKi HumanpIC50 HumanpEC50 Human012345Highcharts.com
DB Assay description Assay Type Standard value Standard parameter Original value Original units Original parameter Reference
2`-deoxynucleoside 5`-phosphate N-hydrolase 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3351218] [UniProtKB: O43598]
ChEMBL Inhibition of human DNPH1 assessed as 2-deoxyribose 5-phosphate production by spectrophotometrically B 4.72 pKi 19200 nM Ki Eur J Med Chem (2014) 85: 418-437 [PMID:25108359]
2`-deoxynucleoside 5`-phosphate N-hydrolase 1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3329079] [UniProtKB: O35820]
ChEMBL Inhibition of rat DNPH1 assessed as 2-deoxyribose 5-phosphate production by spectrophotometrically B 4.4 pKi 40000 nM Ki Eur J Med Chem (2014) 85: 418-437 [PMID:25108359]
A1 receptor/Adenosine A1 receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL226] [GtoPdb: 18] [UniProtKB: P30542]
ChEMBL Agonist activity at human A1AR expressed in HEK293T/17 cells assessed as inhibition of isoproterenol-induced cAMP accumulation incubated for 10 mins by luciferase reporter assay F 6.3 pEC50 500 nM EC50 J Med Chem (2012) 55: 6467-6477 [PMID:22738238]
Adenylate kinase 2 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2376] [UniProtKB: P29410]
ChEMBL Inhibitory activity against rat adenylate kinase II was determined in the presence of AMP B 4.1 pKi 80000 nM Ki J Med Chem (1982) 25: 1179-1184 [PMID:6292418]
protein kinase AMP-activated non-catalytic subunit beta 2/protein kinase AMP-activated non-catalytic subunit gamma 1/protein kinase AMP-activated catalytic subunit alpha 2/protein kinase AMP-activated catalytic subunit alpha 1/protein kinase AMP-activated non-catalytic subunit gamma 3/protein kinase AMP-activated non-catalytic subunit gamma 2/protein kinase AMP-activated non-catalytic subunit beta 1/AMP-activated protein kinase, AMPK in Human (target type: PROTEIN COMPLEX GROUP) [ChEMBL: CHEMBL2096907] [GtoPdb: 1544, 1545, 1542, 1541, 1547, 1546, 1543] [UniProtKB: O43741, P54619, P54646, Q13131, Q9UGI9, Q9UGJ0, Q9Y478]
ChEMBL Activation of human AMPK after 15 mins B 5.23 pEC50 5900 nM EC50 ACS Med Chem Lett (2010) 1: 478-482 [PMID:24900234]
protein kinase AMP-activated non-catalytic subunit gamma 1/protein kinase AMP-activated catalytic subunit alpha 1/protein kinase AMP-activated non-catalytic subunit beta 1/AMP-activated protein kinase (AMPK) alpha-1/beta-1/gamma-1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL2111345] [GtoPdb: 1545, 1541, 1543] [UniProtKB: P54619, Q13131, Q9Y478]
ChEMBL Binding affinity to recombinant human AMPK alpha1/beta1/gamma1 by SPR binding assay B 5.43 pKd 3700 nM Kd J Med Chem (2016) 59: 8068-8081 [PMID:27490827]
ChEMBL Activation of recombinant human AMPK alpha1/beta1/gamma1 using Cy5-labelled SAMS as substrate assessed as protection from Thr172 residue dephosphorylation preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay B 5.82 pEC50 1519 nM EC50 J Med Chem (2016) 59: 8068-8081 [PMID:27490827]
protein kinase AMP-activated non-catalytic subunit gamma 1/protein kinase AMP-activated catalytic subunit alpha 2/protein kinase AMP-activated non-catalytic subunit beta 1/AMPK alpha2/beta1/gamma1 in Human (target type: PROTEIN COMPLEX) [ChEMBL: CHEMBL3038455] [GtoPdb: 1545, 1542, 1543] [UniProtKB: P54619, P54646, Q9Y478]
ChEMBL Allosteric activation of AMPK alpha2/beta1/gamma1 in human HepG2 cells by scintillation proximity assay B 5.85 pEC50 1400 nM EC50 Bioorg Med Chem Lett (2020) 30: 126790-126790 [PMID:31744674]
Fructose-1,6-bisphosphatase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3975] [UniProtKB: P09467]
ChEMBL Inhibition of human liver fructose-1,6-bisphosphatase expressed in Escherichia coli BL21 (DE3) assessed as reduction of NADP+ to NADPH by phosphoglucose isomerase/glucose-6-phosphate dehydrogenase coupled assay B 5.01 pIC50 9800 nM IC50 Eur J Med Chem (2010) 45: 1478-1484 [PMID:20116906]
ChEMBL Inhibition of human liver FBPase expressed in Escherichia coli BL21(DE3) using FBP as substrate in presence of PGI and G6PDH by spectrophotometric method B 5.48 pIC50 3300 nM IC50 J Med Chem (2020) 63: 10307-10329 [PMID:32820629]
ChEMBL Inhibition of human recombinant FBPase expressed in Escherichia coli BL21(DE3) by phosphoglucose isomerase and glucose-6-phosphate dehydrogenase coupled assay B 5.49 pIC50 3200 nM IC50 Bioorg Med Chem (2014) 22: 1850-1862 [PMID:24530031]
ChEMBL Inhibition of human liver FBPase expressed in Escherichia coli BL21(DE3) Rosetta cells assessed as reduction of NADP+ to NADPH by phosphoglucose isomerase and glucose-6-phosphate dehydrogenase coupling based spectrophotometry B 5.49 pIC50 3200 nM IC50 Eur J Med Chem (2015) 90: 394-405 [PMID:25461330]
ChEMBL Inhibition of human liver FBP1 incubated for 5 mins by fluorescence method B 5.59 pIC50 2600 nM IC50 J Nat Prod (2020) 83: 1541-1552 [PMID:32364726]
ChEMBL Inhibition of wild-type full-length human liver FBPase expressed in Escherichia coli BL21 (DE3) using FBP as substrate by malachite green dye based assay B 5.64 pIC50 2300 nM IC50 Eur J Med Chem (2020) 203: 112500-112500 [PMID:32711108]
ChEMBL Inhibition of human FBPase expressed in Escherichia coli BL21 (DE3) using FBP as substrate incubated for 5 mins by malachite green dye based spectrophotometry B 5.64 pIC50 2300 nM IC50 Eur J Med Chem (2019) 184: 111749-111749 [PMID:31589992]
ChEMBL Inhibition of human liver FBPase expressed in Escherichia coli by spectrophotometry method B 6 pIC50 1000 nM IC50 Eur J Med Chem (2017) 141: 473-505 [PMID:29055870]
ChEMBL Inhibition of human liver FBPase expressed in Escherichia coli by spectrophotometry B 6 pIC50 1000 nM IC50 J Med Chem (2009) 52: 2880-2898 [PMID:19348494]
ChEMBL Inhibition of FBPase (unknown origin) B 6 pIC50 1000 nM IC50 Medchemcomm (2011) 2: 287-290
ChEMBL Concentration required to inhibit the human liver recombinant fructose-1,6-bisphosphatase. B 6.1 pIC50 800 nM IC50 Bioorg Med Chem Lett (2001) 11: 17-21 [PMID:11140724]
ChEMBL Inhibition of FBPase in human liver B 6.36 pIC50 440 nM IC50 Bioorg Med Chem Lett (2010) 20: 594-599 [PMID:19969452]
ChEMBL Inhibition of FBPase in human liver using fructose-2,6phosphate as a substrate B 6.85 pIC50 140 nM IC50 Bioorg Med Chem Lett (2010) 20: 594-599 [PMID:19969452]
Fructose-1,6-bisphosphatase in Pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2263] [UniProtKB: P00636]
ChEMBL Inhibition of pig FBPase expressed in Escherichia coli EK1601 by spectrophotometry B 5.89 pIC50 1300 nM IC50 Bioorg Med Chem (2009) 17: 3916-3922 [PMID:19419876]
ChEMBL Inhibition of pig kidney fructose-1,6-bisphosphatase expressed in Escherichia coli BL21 (DE3) assessed as reduction of NADP+ to NADPH by phosphoglucose isomerase/glucose-6-phosphate dehydrogenase coupled assay B 5.89 pIC50 1300 nM IC50 Eur J Med Chem (2010) 45: 1478-1484 [PMID:20116906]
Human immunodeficiency virus type 1 protease in Human immunodeficiency virus 1 (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL243] [UniProtKB: Q72874]
ChEMBL Equilibrium constant for the interaction between inhibitor and HIV-1 Protease B 8.95 pKd 1.13 nM Kd J Med Chem (2002) 45: 5430-5439 [PMID:12459011]
ChEMBL Inhibition constant against HIV-1 Protease B 9.64 pKi 0.23 nM Ki J Med Chem (2002) 45: 5430-5439 [PMID:12459011]
Ketopantoate reductase in Escherichia coli (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4855] [UniProtKB: P0A9J4]
ChEMBL Binding affinity to Escherichia coli KPR B 2.3 pKd >5000000 nM Kd J Med Chem (2006) 49: 4992-5000 [PMID:16884311]
ChEMBL Inhibition of Escherichia coli KPR B 2.2 pKi 6300000 nM Ki J Med Chem (2006) 49: 4992-5000 [PMID:16884311]
L-lactate dehydrogenase A chain in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4835] [UniProtKB: P00338]
ChEMBL Binding affinity to 6-His-tagged human LDHA (2 to 322 amino acid residue) expressed in Escherichia coli BL21 (DE3) cells by NMR analysis in presence of NADH B 3.19 pKd 650000 nM Kd J Med Chem (2012) 55: 3285-3306 [PMID:22417091]
ChEMBL Inhibition of full length human recombinant LDHA expressed in Escherichia coli BL21 (DE3) cells after 10 mins by spectrophotometric analysis B 3.3 pIC50 >500000 nM IC50 J Med Chem (2012) 55: 3285-3306 [PMID:22417091]
P2Y purinoceptor 1 in Turkey (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5720] [UniProtKB: P49652]
ChEMBL Measure of Agonist Potency at turkey Purinoceptor P2Y1 stably expressed in 131N1 astrocytoma cell F 5.46 pEC50 3500 nM EC50 J Med Chem (2002) 45: 208-218 [PMID:11754592]
ChEMBL Measure of Agonist Potency at turkey Purinoceptor P2Y1 stably expressed in 131N1 astrocytoma cell F 5.55 pEC50 2800 nM EC50 J Med Chem (2002) 45: 208-218 [PMID:11754592]
P2Y1 receptor/Purinergic receptor P2Y1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4315] [GtoPdb: 323] [UniProtKB: P47900]
ChEMBL Measure of Agonist Potency at human P2Y purinoceptor 1 (hP2Y1) stably expressed in 131N1 astrocytoma cell F 5.14 pEC50 7200 nM EC50 J Med Chem (2002) 45: 208-218 [PMID:11754592]
ChEMBL Measure of Agonist Potency at human P2Y purinoceptor 1 (hP2Y1) stably expressed in 131N1 astrocytoma cell F 5.82 pEC50 1500 nM EC50 J Med Chem (2002) 45: 208-218 [PMID:11754592]
P2Y11 receptor/Purinergic receptor P2Y11 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4867] [GtoPdb: 327] [UniProtKB: Q96G91]
ChEMBL Measure of Agonist Potency at human P2Y purinoceptor 11 (hP2Y11) stably expressed in 131N1 astrocytoma cell at 10 uM F 4.76 pEC50 17250 nM EC50 J Med Chem (2002) 45: 208-218 [PMID:11754592]
P2Y2 receptor/Purinergic receptor P2Y2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4398] [GtoPdb: 324] [UniProtKB: P41231]
ChEMBL Measure of Agonist Potency at human P2Y purinoceptor 2 (hP2Y2) stably expressed in 131N1 astrocytoma cell F 5.43 pEC50 3700 nM EC50 J Med Chem (2002) 45: 208-218 [PMID:11754592]
ChEMBL Measure of Agonist Potency at human P2Y purinoceptor 2 (hP2Y2) stably expressed in 131N1 astrocytoma cell F 7.07 pEC50 85 nM EC50 J Med Chem (2002) 45: 208-218 [PMID:11754592]
TRPM2/Transient receptor potential cation channel subfamily M member 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1250402] [GtoPdb: 494] [UniProtKB: O94759]
ChEMBL Inhibition of TRPM2 (unknown origin) B 4.15 pIC50 70000 nM IC50 J Med Chem (2013) 56: 10079-10102 [PMID:24304219]
ChEMBL Inhibition of human TRPM2 assessed as reduction in ADPR-induced channel currents by whole cell patch clamp electrophysiology method B 4.15 pIC50 70000 nM IC50 Eur J Med Chem (2018) 152: 235-252 [PMID:29723786]
SRC proto-oncogene, non-receptor tyrosine kinase/Tyrosine-protein kinase SRC in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL267] [GtoPdb: 2206] [UniProtKB: P12931]
ChEMBL Inhibition of p60c-src expressed in chick embryo fibroblast B 7 pIC50 100 nM IC50 J Nat Prod (1992) 55: 1529-1560 [PMID:1479375]
TRPM4 in Human [GtoPdb: 496] [UniProtKB: Q8TD43]
GtoPdb - - 4.7 pIC50 - - - Pflugers Arch (2004) 448: 70-5 [PMID:14758478]
AMP kinase in Human [GtoPdb: 1540]
GtoPdb Activation of AMPK partially purified from HEK cells. - 4.21 pEC50 61000 nM EC50 Cell Metab (2006) 3: 403-16 [PMID:16753576]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]