ChEMBL ligand: CHEMBL233248 (Allyl isothiocyanate, Allyl isothiocyanate, stabilized, FEMA NO. 2034, Mustard oil, NSC-5572, Oil, mustard, Oil,mustard)
Macrophage migration inhibitory factor in Human [ChEMBL: CHEMBL2085] [UniProtKB: P14174] There should be some charts here, you may need to enable JavaScript!
TRPA1/Transient receptor potential cation channel subfamily A member 1 in Human [ChEMBL: CHEMBL6007] [GtoPdb: 485] [UniProtKB: O75762] TRPA1/Transient receptor potential cation channel subfamily A member 1 in Mouse [ChEMBL: CHEMBL1075310] [GtoPdb: 485] [UniProtKB: Q8BLA8] TRPA1/Transient receptor potential cation channel subfamily A member 1 in Rat [ChEMBL: CHEMBL5160] [GtoPdb: 485] [UniProtKB: Q6RI86] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Macrophage migration inhibitory factor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2085] [UniProtKB: P14174]
ChEMBL	Inhibition of human recombinant macrophage migration inhibitory factor tautomerase activity expressed in Escherichia coli DH5alpha using L-dopachrome methyl ester as substrate incubated for 5 mins prior to substrate addition measured for 2 mins by spectrophotometric analysis	B	5.96	pIC50	1100	nM	IC50	Eur J Med Chem (2015) 93: 501-510 [PMID:25743213]
ChEMBL	Inhibition of macrophage migration inhibitory factor tautomerase activity in human Jurkat T cells using L-dopachrome methyl ester as substrate incubated for 30 mins prior to substrate addition measured for 2 mins by spectrophotometric analysis	B	6.6	pIC50	250	nM	IC50	Eur J Med Chem (2015) 93: 501-510 [PMID:25743213]
TRPA1/Transient receptor potential cation channel subfamily A member 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL6007] [GtoPdb: 485] [UniProtKB: O75762]
GtoPdb	-	-	4.2	pEC50	63100	nM	EC50	Proc Natl Acad Sci USA (2006) 103: 19564-8 [PMID:17164327]
ChEMBL	Activation of TRPA1	B	4.47	pEC50	33500	nM	EC50	Nature (2007) 445: 541-545 [PMID:17237762]
ChEMBL	Agonist activity at human TRPA1 expressed in T-REx-HEK293 cells at -75 mV holding potential by patch-clamp electrophysiology	F	4.65	pEC50	22387.21	nM	EC50	J Med Chem (2010) 53: 7011-7020 [PMID:20806939]
ChEMBL	Agonist activity at human TRPA1 expressed in T-REx-HEK293 cells at -30 mV holding potential by patch-clamp electrophysiology	F	4.92	pEC50	12022.64	nM	EC50	J Med Chem (2010) 53: 7011-7020 [PMID:20806939]
ChEMBL	Agonist activity at human TRPA1 expressed in T-REx-HEK293 cells at 75 mV holding potential by patch-clamp electrophysiology	F	4.98	pEC50	10471.29	nM	EC50	J Med Chem (2010) 53: 7011-7020 [PMID:20806939]
ChEMBL	Agonist activity at TRPA1 (unknown origin)	B	5.09	pEC50	8120	nM	EC50	Bioorg Med Chem Lett (2020) 30: 127142-127142 [PMID:32249116]
ChEMBL	Agonist activity at human TRPA1 expressed in T-REx-HEK293 cells assessed as time point of maximal current amplitude by patch-clamp electrophysiology	F	5.24	pEC50	5754.4	nM	EC50	J Med Chem (2010) 53: 7011-7020 [PMID:20806939]
ChEMBL	Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay	B	5.36	pEC50	4365.16	nM	EC50	Eur J Med Chem (2019) 170: 141-156 [PMID:30878828]
ChEMBL	Agonist activity at human TRPA1 expressed in HEK293 cells assessed as increase in calcium influx by Fluo-4-AM dye based fluorescence assay	B	5.4	pEC50	4000	nM	EC50	Eur J Med Chem (2019) 170: 141-156 [PMID:30878828]
ChEMBL	Activation of human TRPA1 expressed in HEK293 cells assessed as increase in calcium level by fluo-4 dye based plate reader analysis	B	5.41	pEC50	3900	nM	EC50	J Nat Prod (2016) 79: 697-703 [PMID:26905390]
ChEMBL	Activation of TRPA1 channel	B	5.72	pEC50	1900	nM	EC50	J Med Chem (2010) 53: 5085-5107 [PMID:20356305]
ChEMBL	Agonist activity at human TRPA1 channel expressed in HEK293 cells assessed as calcium influx measured at every 1 second for 60 seconds by fluorescence analysis	F	5.95	pEC50	1120	nM	EC50	Bioorg Med Chem Lett (2011) 21: 4857-4859 [PMID:21741838]
ChEMBL	Activation of human TRPA1 expressed in TREx-HEK cells by Fluo-4 AM dye-based Ca2+ imaging assay	B	6.24	pEC50	580	nM	EC50	J Nat Prod (2015) 78: 1937-1941 [PMID:26263397]
ChEMBL	Agonist activity at TRPA1 isolated from human W138 cells expressed in HEK293T cells assessed as intracellular Ca2+ influx by Fluo-4 AM staining-based fluorescence analysis	B	6.3	pEC50	500	nM	EC50	J Nat Prod (2014) 77: 285-297 [PMID:24484240]
ChEMBL	Agonist activity at human TRPA1 expressed in T-REx-HEK293 cells assessed as increase of intracellular calcium level by FDSS assay	F	6.4	pEC50	398	nM	EC50	J Med Chem (2010) 53: 7011-7020 [PMID:20806939]
TRPA1/Transient receptor potential cation channel subfamily A member 1 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075310] [GtoPdb: 485] [UniProtKB: Q8BLA8]
ChEMBL	Agonist activity at mouse TRPA1 expressed in CHO cells assessed as increase in calcium influx by Fluo-3 based FLIPR analysis	B	4.66	pEC50	22000	nM	EC50	Eur J Med Chem (2019) 170: 141-156 [PMID:30878828]
GtoPdb	-	-	4.7	pEC50	-	-	-	Neuron (2004) 41: 849-57 [PMID:15046718]
TRPA1/Transient receptor potential cation channel subfamily A member 1 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5160] [GtoPdb: 485] [UniProtKB: Q6RI86]
ChEMBL	Antagonist activity against rat recombinant TRPA1 expressed in HEK293 cells assessed as inhibition of AITC-induced Ca2+ influx pre-treated 5 mins before capsaicin addition by Fluo-4 dye based assay	B	4.07	pIC50	85900	nM	IC50	Bioorg Med Chem Lett (2015) 25: 1009-1011 [PMID:25666822]
ChEMBL	Antagonist activity at rat recombinant TRPA1 expressed in HEK293 cells assessed as desensitization by measuring inhibition of allyl isothiocyanate induced intracellular calcium accumulation preincubated for 5 mins followed by allyl isothiocyanate stimulation by Fluo4-AM based assay	B	5.77	pIC50	1710	nM	IC50	J Med Chem (2023) 66: 6994-7015 [PMID:37192374]
GtoPdb	-	-	5	pEC50	-	-	-	Nature (2004) 427: 260-5 [PMID:14712238]
ChEMBL	Agonist activity at rat TRPA1 channel expressed in HEK293 cells assessed as calcium influx measured at every 1 second for 60 seconds by fluorescence analysis	F	5.24	pEC50	5813	nM	EC50	Bioorg Med Chem Lett (2011) 21: 4857-4859 [PMID:21741838]
ChEMBL	Agonist activity at rat TRPA1 ion channel expressed in HEK293 cells assessed as calcium influx by fluo-4-Am-based fluorimetry	F	5.6	pEC50	2500	nM	EC50	Bioorg Med Chem Lett (2012) 22: 1674-1677 [PMID:22257892]
ChEMBL	Agonist activity at rat recombinant TRPA1 expressed in HEK293 cells assessed as Ca2+ influx by Fluo-4 dye based assay	B	5.7	pEC50	2000	nM	EC50	Bioorg Med Chem Lett (2015) 25: 1009-1011 [PMID:25666822]
ChEMBL	Agonist activity at rat recombinant TRPA1 expressed in HEK293 cells assessed as increase in intracellular calcium accumulation by Fluo4-AM based assay	B	5.85	pEC50	1410	nM	EC50	J Med Chem (2023) 66: 6994-7015 [PMID:37192374]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]