ChEMBL ligand: CHEMBL469912 (3123-L, 3123L, CL 13,900, CL-13900, GNF-PF-2016, P-638, Puromicina, Puromycin, Puromycine, TCMDC-123493)
Aminopeptidase N in Human [ChEMBL: CHEMBL1907] [GtoPdb: 1560] [UniProtKB: P15144] Aminopeptidase N in Pig [ChEMBL: CHEMBL2590] [UniProtKB: P15145] There should be some charts here, you may need to enable JavaScript!
Plasmodium falciparum [ChEMBL: CHEMBL364] There should be some charts here, you may need to enable JavaScript!
Plasmodium vivax [ChEMBL: CHEMBL613013] There should be some charts here, you may need to enable JavaScript!
Puromycin-sensitive aminopeptidase in Human [ChEMBL: CHEMBL2264] [GtoPdb: 1575] [UniProtKB: P55786] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
Aminopeptidase N in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1907] [GtoPdb: 1560] [UniProtKB: P15144]
ChEMBL	Inhibition of recombinant human C-terminal His10-tagged APN (Lys69 to Lys967 residues) using Ala-AMC as substrate measured for 30 mins by fluorescence assay	B	4.39	pIC50	41000	nM	IC50	Eur J Med Chem (2017) 139: 325-336 [PMID:28803047]
Aminopeptidase N in Pig (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2590] [UniProtKB: P15145]
ChEMBL	Inhibition of porcine aminopeptidase-N using Leu-pNA as substrate after 30 mins	B	4	pIC50	>100000	nM	IC50	Eur J Med Chem (2015) 106: 26-33 [PMID:26513642]
Plasmodium falciparum (target type: ORGANISM) [ChEMBL: CHEMBL364]
ChEMBL	Antimalarial activity against Plasmodium falciparum 3D7 infected HEK293 cells by DAPI-staining based assay	F	6.39	pIC50	410	nM	IC50	J Med Chem (2019) 62: 2485-2498 [PMID:30715882]
ChEMBL	Antigametocytocidal activity against Plasmodium falciparum NF54-pfs16-GPF late (4 to 6) gametocyte assessed as viability after 72 hrs by MitoTracker Red CM-H2XRos/micro-plate confocal imaging system	F	6.71	pIC50	194	nM	IC50	Bioorg Med Chem Lett (2015) 25: 327-332 [PMID:25488841]
ChEMBL	Antigametocytocidal activity against Plasmodium falciparum NF54-pfs16-GPF early (1 to 3) gametocyte assessed as viability after 72 hrs by MitoTracker Red CM-H2XRos/micro-plate confocal imaging system	F	6.76	pIC50	175	nM	IC50	Bioorg Med Chem Lett (2015) 25: 327-332 [PMID:25488841]
ChEMBL	Antimalarial activity against synchronous ring stage of Plasmodium falciparum 3D7 assessed as parasite growth inhibition incubated for 72 hrs by Griffith assay based fluorescence analysis	F	6.83	pIC50	148.9	nM	IC50	Eur J Med Chem (2021) 221: 113518-113518 [PMID:34058708]
ChEMBL	Antimalarial activity against early (1 to 3) gametocytic stage of Plasmodium falciparum after 72 hrs by image-based HTS assay	F	6.86	pIC50	137	nM	IC50	J Med Chem (2013) 56: 7727-7740 [PMID:23927763]
ChEMBL	Antimalarial activity against synchronous ring stage of Plasmodium falciparum Dd2 assessed as parasite growth inhibition incubated for 72 hrs by Griffith assay based fluorescence analysis	F	6.94	pIC50	114.4	nM	IC50	Eur J Med Chem (2021) 221: 113518-113518 [PMID:34058708]
ChEMBL	Antimalarial activity against Plasmodium falciparum 3D7 after 72 hrs by DAPI staining-based confocal imaging analysis	F	6.96	pIC50	110.8	nM	IC50	J Med Chem (2019) 62: 622-640 [PMID:30537832]
ChEMBL	Antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum 3D7 ring stage incubated for 72 hrs by DAPI-staining based imaging analysis	F	6.96	pIC50	110	nM	IC50	J Nat Prod (2020) 83: 3435-3444 [PMID:33105995]
ChEMBL	Antimalarial activity against Plasmodium falciparum Dd2 after 72 hrs by DAPI staining-based confocal imaging analysis	F	6.97	pIC50	106.6	nM	IC50	J Med Chem (2019) 62: 622-640 [PMID:30537832]
ChEMBL	Antiplasmodial activity against ring stage drug-sensitive Plasmodium falciparum 3D7 assessed as inhibition of parasite growth incubated for 72 hrs by DAPI staining based assay	F	7.04	pIC50	91	nM	IC50	J Nat Prod (2023) 86: 2171-2184 [PMID:37610242]
ChEMBL	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum 3D7 assessed as reduction in parasite replication incubated for 62 hrs	F	7.05	pIC50	89	nM	IC50	J Nat Prod (2012) 75: 1206-1209 [PMID:22680914]
ChEMBL	Antimalarial activity against multidrug resistant Plasmodium falciparum Dd2 assessed as parasite growth inhibition incubated for 72 hrs by DAPI staining method	F	7.09	pIC50	81.4	nM	IC50	J Nat Prod (2023) 86: 557-565 [PMID:36799121]
ChEMBL	Antiplasmodial activity against Plasmodium falciparum 3D7 incubated for 72 hrs by DAPI staining based analysis	F	7.1	pIC50	80	nM	IC50	J Nat Prod (2023) 86: 1317-1334 [PMID:37171174]
ChEMBL	Antiplasmodial activity against drug-resistant Plasmodium falciparum Dd2 ring stage forms assessed as inhibition of parasite growth after 72 hrs by DAPI staining-based confocal microscopic analysis	F	7.13	pIC50	74.7	nM	IC50	J Nat Prod (2019) 82: 1019-1023 [PMID:30865443]
ChEMBL	Antiplasmodial activity against drug-sensitive Plasmodium falciparum 3D7 ring stage forms assessed as inhibition of parasite growth after 72 hrs by DAPI staining-based confocal microscopic analysis	F	7.15	pIC50	70.2	nM	IC50	J Nat Prod (2019) 82: 1019-1023 [PMID:30865443]
ChEMBL	Antiplasmodial activity against chloroquine-resistant Plasmodium falciparum Dd2 ring stage incubated for 72 hrs by DAPI-staining based imaging analysis	F	7.17	pIC50	68	nM	IC50	J Nat Prod (2020) 83: 3435-3444 [PMID:33105995]
ChEMBL	Antimalarial activity against asexual stage of Plasmodium falciparum 3D7 after 72 hrs by image-based HTS assay	F	7.19	pIC50	64	nM	IC50	J Med Chem (2013) 56: 7727-7740 [PMID:23927763]
ChEMBL	Antimalarial activity against Plasmodium falciparum Dd2 incubated for 72 hours by DAPI-staining based confocal imaging analysis	F	7.21	pIC50	62	nM	IC50	Bioorg Med Chem Lett (2016) 26: 3326-3329 [PMID:27212070]
ChEMBL	Antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum 3D7 measured after 72 hrs by DAPI staining based high throughput screening assay	F	7.21	pIC50	62	nM	IC50	Bioorg Med Chem Lett (2017) 27: 2602-2607 [PMID:28400231]
ChEMBL	Antiplasmodial activity against chloroquine-resistant Plasmodium falciparum Dd2 measured after 72 hrs by DAPI staining based high throughput screening assay	F	7.21	pIC50	61	nM	IC50	Bioorg Med Chem Lett (2017) 27: 2602-2607 [PMID:28400231]
ChEMBL	Antiplasmodial activity against 4-aminoquinoline/antifolates-resistant Plasmodium falciparum Dd2 ring stage infected in human erythrocytes after 72 hrs by DAPI staining based method	F	7.21	pIC50	61	nM	IC50	J Nat Prod (2018) 81: 1588-1597 [PMID:29969262]
ChEMBL	Antiplasmodial activity against drug-sensitive Plasmodium falciparum 3D7 ring stage infected in human erythrocytes after 72 hrs by DAPI staining based method	F	7.22	pIC50	60	nM	IC50	J Nat Prod (2018) 81: 1588-1597 [PMID:29969262]
ChEMBL	Antimalarial activity against Plasmodium falciparum 3D7 incubated for 72 hours by DAPI-staining based confocal imaging analysis	F	7.22	pIC50	60	nM	IC50	Bioorg Med Chem Lett (2016) 26: 3326-3329 [PMID:27212070]
ChEMBL	Antimalarial activity against Plasmodium falciparum 3D7 after 72 hrs by DAPI staining-based fluorescence microscopic analysis	F	7.27	pIC50	53.2	nM	IC50	Bioorg Med Chem Lett (2017) 27: 4091-4095 [PMID:28774427]
ChEMBL	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum 3D7 after 72 hrs by DAPI staining based confocal microplate imaging method	F	7.28	pIC50	53	nM	IC50	J Nat Prod (2017) 80: 3211-3217 [PMID:29236492]
ChEMBL	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum 3D7 after 72 hrs by DAPI staining based fluorescence assay	F	7.28	pIC50	53	nM	IC50	J Nat Prod (2018) 81: 1079-1083 [PMID:29533611]
ChEMBL	Antimalarial activity against late (4 to 5) gametocytic stage of Plasmodium falciparum after 72 hrs by image-based HTS assay	F	7.34	pIC50	46	nM	IC50	J Med Chem (2013) 56: 7727-7740 [PMID:23927763]
ChEMBL	Antiplasmodial activity against chloroquine-resistant Plasmodium falciparum Dd2 assessed as inhibition of parasite growth after 72 hrs by DAPI staining-based confocal microscopic analysis	F	7.34	pIC50	45.2	nM	IC50	J Nat Prod (2015) 78: 2932-2939 [PMID:26651537]
ChEMBL	Antimalarial activity against chloroquine-resistant Plasmodium falciparum Dd2 assessed as reduction in parasite growth incubated for 72 hrs by DAPI staining based fluorescence assay	F	7.35	pIC50	45	nM	IC50	J Nat Prod (2020) 83: 316-322 [PMID:32067457]
ChEMBL	Antimalarial activity against chloroquine-resistant Plasmodium falciparum Dd2 infected in human RBC after 72 hrs by DAPI staining based confocal microscopic method	F	7.35	pIC50	45	nM	IC50	J Nat Prod (2017) 80: 114-125 [PMID:28001067]
ChEMBL	Antimalarial activity against chloroquine-resistant Plasmodium falciparum Dd2 after 72 hrs by DAPI staining based fluorescence assay	F	7.36	pIC50	44	nM	IC50	J Nat Prod (2018) 81: 1079-1083 [PMID:29533611]
ChEMBL	Antimalarial activity against Plasmodium falciparum Dd2 after 72 hrs by DAPI staining-based fluorescence microscopic analysis	F	7.36	pIC50	43.8	nM	IC50	Bioorg Med Chem Lett (2017) 27: 4091-4095 [PMID:28774427]
ChEMBL	Antimalarial activity against chloroquine sensitive Plasmodium falciparum 3D7 assessed as parasite growth inhibition incubated for 72 hrs by DAPI staining method	F	7.37	pIC50	42.9	nM	IC50	J Nat Prod (2023) 86: 557-565 [PMID:36799121]
ChEMBL	Antimalarial activity against Plasmodium falciparum W2 assessed as inhibition of parasite growth	F	7.38	pIC50	42	nM	IC50	Bioorg Med Chem (2015) 23: 5120-5130 [PMID:25913864]
ChEMBL	Antiplasmodial activity against choloroquine-sensitive ring stage of Plasmodium falciparum 3D7 assessed as inhibition of parasite growth measured after 72 hrs by DAPI staining based analysis	F	7.48	pIC50	33	nM	IC50	J Nat Prod (2024) 87: 849-854 [PMID:38416027]
ChEMBL	Antimalarial activity against chloroquine-resistant Plasmodium falciparum Dd2 after 72 hrs by DAPI staining based confocal microplate imaging method	F	7.49	pIC50	32	nM	IC50	J Nat Prod (2017) 80: 3211-3217 [PMID:29236492]
ChEMBL	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum 3D7 infected in human RBC after 72 hrs by DAPI staining based confocal microscopic method	F	7.64	pIC50	23	nM	IC50	J Nat Prod (2017) 80: 114-125 [PMID:28001067]
ChEMBL	Antimalarial activity against chloroquine-sensitive Plasmodium falciparum 3D7 assessed as reduction in parasite rowth incubated for 72 hrs by DAPI staining based fluorescence assay	F	7.64	pIC50	23	nM	IC50	J Nat Prod (2020) 83: 316-322 [PMID:32067457]
ChEMBL	Antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum 3D7 assessed as inhibition of parasite growth after 72 hrs by DAPI staining-based confocal microscopic analysis	F	7.64	pIC50	22.7	nM	IC50	J Nat Prod (2015) 78: 2932-2939 [PMID:26651537]
ChEMBL	NOVARTIS: Inhibition of Plasmodium falciparum 3D7 (drug-susceptible) proliferation in erythrocyte-based infection assay	F	6.21	pEC50	611	nM	EC50	Proc Natl Acad Sci U S A (2008) 105: 9059-9064 [PMID:18579783]
ChEMBL	NOVARTIS: Inhibition of Plasmodium falciparum W2 (drug-resistant) proliferation in erythrocyte-based infection assay	F	6.48	pEC50	328	nM	EC50	Proc Natl Acad Sci U S A (2008) 105: 9059-9064 [PMID:18579783]
Plasmodium vivax (target type: ORGANISM) [ChEMBL: CHEMBL613013]
ChEMBL	Antimalarial activity against liver stage Plasmodium vivax schizont infected in human hepatocytes preincubated for 5 to 10 days followed by compound addition and measured after 12 days post infection by immunofluorescence staining based assay	F	5.64	pEC50	2290.87	nM	EC50	J Nat Prod (2024) 87: 315-321 [PMID:38262446]
ChEMBL	Antimalarial activity against liver stage Plasmodium vivax schizont infected in human hepatocytes preincubated for 5 to 10 days followed by compound addition and measured after 12 days post infection by immunofluorescence staining based assay	F	5.64	pEC50	2290	nM	EC50	J Nat Prod (2024) 87: 315-321 [PMID:38262446]
ChEMBL	Antimalarial activity against liver stage Plasmodium vivax hypnozoite infected in human hepatocytes preincubated for 5 to 10 days followed by compound addition and measured after 12 days post infection by immunofluorescence staining based assay	F	6.11	pEC50	776.25	nM	EC50	J Nat Prod (2024) 87: 315-321 [PMID:38262446]
ChEMBL	Antimalarial activity against liver stage Plasmodium vivax hypnozoite infected in human hepatocytes preincubated for 5 to 10 days followed by compound addition and measured after 12 days post infection by immunofluorescence staining based assay	F	6.11	pEC50	776	nM	EC50	J Nat Prod (2024) 87: 315-321 [PMID:38262446]
Puromycin-sensitive aminopeptidase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2264] [GtoPdb: 1575] [UniProtKB: P55786]
ChEMBL	Inhibition of recombinant human N-terminal His6-MBP-tagged PSA expressed in Escherichia coli BL21 STAR (DE3) using 4-Ala-MNA as substrate measured for 30 mins by thefluorescence assay	B	5.01	pIC50	9700	nM	IC50	Eur J Med Chem (2017) 139: 325-336 [PMID:28803047]
GtoPdb	-	-	5.61	pIC50	2450	nM	IC50	Eur J Med Chem (2015) 106: 26-33 [PMID:26513642]
ChEMBL	Inhibition of recombinant human puromycin sensitive aminopeptidase using Leu-pNA as substrate after 30 mins	B	5.61	pIC50	2450	nM	IC50	Eur J Med Chem (2015) 106: 26-33 [PMID:26513642]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]