ChEMBL ligand: CHEMBL376756 (Human metastin 45-54, Kisspeptin-10, Kisspeptin-10 (human))
kisspeptin receptor/Metastin receptor in Human [ChEMBL: CHEMBL5413] [GtoPdb: 266] [UniProtKB: Q969F8] kisspeptin receptor/Metastin receptor in Rat [ChEMBL: CHEMBL1169599] [GtoPdb: 266] [UniProtKB: Q924U1] There should be some charts here, you may need to enable JavaScript!
NPFF1 receptor/Neuropeptide FF receptor 1 in Human [ChEMBL: CHEMBL5951] [GtoPdb: 300] [UniProtKB: Q9GZQ6] There should be some charts here, you may need to enable JavaScript!
NPFF2 receptor/Neuropeptide FF receptor 2 in Human [ChEMBL: CHEMBL5952] [GtoPdb: 301] [UniProtKB: Q9Y5X5] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
kisspeptin receptor/Metastin receptor in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5413] [GtoPdb: 266] [UniProtKB: Q969F8]
ChEMBL	Displacement of [125]metastin from metastin receptor	B	8.89	pKi	1.3	nM	Ki	J Med Chem (2007) 50: 462-471 [PMID:17266198]
ChEMBL	Displacement of [125I]-Kp-10 from human Kiss1 receptor by TopCount scintillation counting method	B	10.21	pKi	0.06	nM	Ki	J Med Chem (2021) 64: 7555-7564 [PMID:34008968]
GtoPdb	-	-	10.4	pKi	-	-	-	Nature (2001) 411: 613-7 [PMID:11385580]; J Biol Chem (2001) 276: 34631-6 [PMID:11457843]
ChEMBL	Binding affinity to human KISS1R expressed in CHO cell membranes	B	10.41	pKi	0.04	nM	Ki	J Med Chem (2013) 56: 8298-8307 [PMID:24047141]
ChEMBL	Displacement of radioligand from human KISS1R transfected in CHO cells	B	10.46	pKi	0.04	nM	Ki	J Med Chem (2014) 57: 6105-6115 [PMID:24918545]
ChEMBL	Inhibition of human KISS1R expressed in CHO cells assessed as inhibition of KISS1R-mediated cell growth after 4 days by cell counting kit-8 assay	B	5.07	pIC50	8510	nM	IC50	J Med Chem (2013) 56: 8298-8307 [PMID:24047141]
ChEMBL	Displacement of [125I]kisspeptin-15 from GPR54	B	9.92	pIC50	0.12	nM	IC50	ACS Med Chem Lett (2011) 2: 53-57 [PMID:24900254]
ChEMBL	Binding affinity to human KISS1R	B	10.09	pIC50	0.08	nM	IC50	Bioorg Med Chem Lett (2012) 22: 6391-6396 [PMID:22975302]
ChEMBL	Displacement of [125I]kisspeptin-15 from human GPR54 expressed in CHO cell membrane	B	10.15	pIC50	0.07	nM	IC50	J Med Chem (2008) 51: 7645-7649 [PMID:19007202]
ChEMBL	Activity at metastin receptor expressed in HEK293 cells by measuring calcium release	F	7.76	pEC50	17.3	nM	EC50	J Med Chem (2007) 50: 462-471 [PMID:17266198]
ChEMBL	Agonist activity at human KISS1R expressed in CHO/dhfr cells assessed as increase in intracellular Ca2+ levels measured for 180 secs by Fluo 3-AM dye based FLIPR assay	F	9.02	pEC50	0.96	nM	EC50	J Med Chem (2016) 59: 8804-8811 [PMID:27589480]
ChEMBL	Agonist activity at human KISS1R transfected in CHO cells assessed as intracellular calcium flux after 24 hrs by functional FLIPR assay	F	9.02	pEC50	0.96	nM	EC50	J Med Chem (2014) 57: 6105-6115 [PMID:24918545]
ChEMBL	Agonist activity at human GPR54 expressed in CHO cells assessed as effect on kisspeptin-10-induced response by FLIPR assay	F	9.62	pEC50	0.24	nM	EC50	J Med Chem (2008) 51: 7645-7649 [PMID:19007202]
ChEMBL	Agonist activity at human KISS1R expressed in CHO cells assessed as increase in calcium mobilization measured at 2 secs interval for 2 mins by fluo-4 NW dye based FLIPR assay	F	10.06	pEC50	0.09	nM	EC50	Bioorg Med Chem Lett (2019) 29: 654-658 [PMID:30598349]
ChEMBL	Agonist activity at human OT7T175 assessed as increase in intracellular calcium level by FLIPR assay	F	10.19	pEC50	0.07	nM	EC50	Bioorg Med Chem Lett (2012) 22: 6328-6332 [PMID:22995619]
ChEMBL	Agonist activity at human KISS1R assessed as induction of intracellular calcium mobilization by fluorometric analysis	F	10.31	pEC50	0.05	nM	EC50	Bioorg Med Chem Lett (2012) 22: 6391-6396 [PMID:22975302]
kisspeptin receptor/Metastin receptor in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1169599] [GtoPdb: 266] [UniProtKB: Q924U1]
GtoPdb	-	-	8.8	pKi	-	-	-	J Biol Chem (2001) 276: 34631-6 [PMID:11457843]
ChEMBL	Displacement of radioligand from rat KISS1R transfected in CHO cells	B	10.06	pKi	0.09	nM	Ki	J Med Chem (2014) 57: 6105-6115 [PMID:24918545]
ChEMBL	Binding affinity to rat KISS1R	B	10.24	pKi	0.06	nM	Ki	J Med Chem (2013) 56: 8298-8307 [PMID:24047141]
ChEMBL	Binding affinity to rat KISS1R	B	9.96	pIC50	0.11	nM	IC50	Bioorg Med Chem Lett (2012) 22: 6391-6396 [PMID:22975302]
ChEMBL	Agonist activity at rat KISS1R transfected in CHO cells assessed as intracellular calcium flux after 24 hrs by functional FLIPR assay	F	8.38	pEC50	4.2	nM	EC50	J Med Chem (2014) 57: 6105-6115 [PMID:24918545]
ChEMBL	Agonist activity at rat KISS1R expressed in CHO/dhfr cells assessed as increase in intracellular Ca2+ levels measured for 180 secs by Fluo 3-AM dye based FLIPR assay	F	8.38	pEC50	4.2	nM	EC50	J Med Chem (2016) 59: 8804-8811 [PMID:27589480]
NPFF1 receptor/Neuropeptide FF receptor 1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5951] [GtoPdb: 300] [UniProtKB: Q9GZQ6]
ChEMBL	Displacement of (D-[125I]Tyr1, MePhe3)-NPFF from NPFFR1 after 2 hrs	B	8.33	pIC50	4.7	nM	IC50	ACS Med Chem Lett (2011) 2: 53-57 [PMID:24900254]
NPFF2 receptor/Neuropeptide FF receptor 2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5952] [GtoPdb: 301] [UniProtKB: Q9Y5X5]
ChEMBL	Displacement of (D-[125I]Tyr1, MePhe3)-NPFF from NPFFR2 after 2 hrs	B	7.12	pIC50	76	nM	IC50	ACS Med Chem Lett (2011) 2: 53-57 [PMID:24900254]

ChEMBL data shown on this page come from version 33:

Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]