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ChEMBL ligand: CHEMBL2169920 |
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DB | Assay description | Assay Type | Standard value | Standard parameter | Original value | Original units | Original parameter | Reference |
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SET and MYND domain containing 2/N-lysine methyltransferase SMYD2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2169716] [GtoPdb: 2714] [UniProtKB: Q9NRG4] | ||||||||
ChEMBL | Binding affinity to recombinant human full-length SMYD2 (1 to 433) by ITC analysis | B | 6.3 | pKd | 500 | nM | Kd | J Med Chem (2015) 58: 1596-1629 [PMID:25406853] |
ChEMBL | Competitive binding affinity to full length human SMYD2 amino acid 1 to 433 expressed in Escherichia coli BL21 (DE3) after 90 mins by radioactive filter-binding assay in presence of P53 peptide | B | 6.52 | pKi | 300 | nM | Ki | Eur J Med Chem (2012) 56: 179-194 [PMID:22975593] |
ChEMBL | Inhibition of SMYD2 (unknown origin) expressed in human U2OS cells assessed as reduction in p53 methylation by Western blot analysis | B | 5 | pIC50 | <10000 | nM | IC50 | J Med Chem (2016) 59: 4578-4600 [PMID:27075367] |
ChEMBL | Inhibition of N-terminal 2xc-myc-tagged human SMYD2 transfected in human MDA-MB-231 cells assessed as reduction in AHNAK methylation after 72 hrs by ICW assay | B | 5.54 | pIC50 | 2900 | nM | IC50 | J Med Chem (2016) 59: 4578-4600 [PMID:27075367] |
ChEMBL | Inhibition of full length 6xHis-tagged SMYD2 (unknown origin) expressed in Escherichia coli using Btn-Ahx GSRAHSSHLKSKKGQSTSRH-amide as substrate after 30 mins in presence 3H-SAM of by scintillation proximity assay | B | 6.09 | pIC50 | 812 | nM | IC50 | J Med Chem (2016) 59: 4578-4600 [PMID:27075367] |
ChEMBL | Inhibition of SMYD2 (unknown origin) using biotinylated GSRAHSSHLKSKKGQSTSRH as substrate assessed as incorporation of tritium labeled methyl group from [3H]-SAM to biotinylated peptide substrate after 40 mins by scintillation proximity assay | B | 6.64 | pIC50 | 230 | nM | IC50 | ACS Med Chem Lett (2015) 6: 695-700 [PMID:26101576] |
GtoPdb | - | - | 6.92 | pIC50 | 120 | nM | IC50 | Structure (2011) 19: 1262-73 [PMID:21782458] |
ChEMBL | Inhibition of recombinant full-length human SMYD2 (1 to 433) expressed in Escherichia coli BL21Star(DE3) cells using p53 peptide as substrate after 90 mins by scintillation proximity assay in presence of [3H]-SAM | B | 6.92 | pIC50 | 120 | nM | IC50 | ACS Med Chem Lett (2015) 6: 695-700 [PMID:26101576] |
ChEMBL | Inhibition of full length human SMYD2 amino acid 1 to 433 expressed in Escherichia coli BL21 (DE3) using Biotinaminohexanoyl- GSRAHSSHLKSKKGQSTSRH as substrate after 75 mins by AlphaScreen assay | B | 6.92 | pIC50 | 120 | nM | IC50 | Eur J Med Chem (2012) 56: 179-194 [PMID:22975593] |
ChEMBL | Inhibition of SMYD2 (unknown origin) expressed in Escherichia coli BL21 (DE3) using Biotinaminohexanoyl-GSRAHSSHLKSKKGQSTSRH as substrate after 75 mins by scintillation proximity assay | B | 6.92 | pIC50 | 120 | nM | IC50 | J Med Chem (2016) 59: 4578-4600 [PMID:27075367] |
ChEMBL | Inhibition of recombinant human full-length SMYD2 (1 to 433) using biotin-aminohexanoyl GSRAHSSHLKSKKGQSTSRH as substrate after 75 mins by AlphaScreen assay | B | 6.92 | pIC50 | 120 | nM | IC50 | J Med Chem (2015) 58: 1596-1629 [PMID:25406853] |
ChEMBL data shown on this page come from version 33:
Mendez D, Gaulton A, Bento AP, Chambers J, De Veij M, Félix E, Magariños MP, Mosquera JF, Mutowo P, Nowotka M, Gordillo-Marañón M, Hunter F, Junco L, Mugumbate G, Rodriguez-Lopez M, Atkinson F, Bosc N, Radoux CJ, Segura-Cabrera A, Hersey A, Leach AR. (2019) 'ChEMBL: towards direct deposition of bioassay data' Nucleic Acids Res., 47(D1). DOI: 10.1093/nar/gky1075. [EPMCID:30398643]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]