ChEMBL ligand: CHEMBL521 (Aches-n-pain, Advil, Advil liqui-gels, Advil migraine liqui-gels, Anadin joint pain, Anadin liquifast, Anadin period pain relief, Anadin ultra, Apsifen, Apsifen-f, Arthrofen 200, Arthrofen 400, Arthrofen 600, Brufen ret, Burana, Caldolor, Cap-profen, Children's advil, Children's advil-flavored, Children's elixsure, Children's ibuprofen, Children's motrin, Codafen continus, Co-op, Cuprofen for child, Cuprofen plus, Deep relief, Ebufac, Femafen, Fenbid, Fenbid fte, Fenpaed, Feverfen, Fleximex, IB-100, Ibucalm, Ibuderm, Ibufac, Ibugel, Ibugel fte, Ibular, Ibuleve, Ibumed, Ibumetin, Ibumousse, Ibuprin, Ibuprofen, Ibuprofenum, Ibuprohm, Ibu-slo, Ibuspray, Ibu-tab, Ibu-tab 200, Ibutime, Inabrin, Infant's advil, Inoven, IP-82, Isisfen, Junifen, Junior strength advil, Junior strength ibuprofen, Junior strength motrin, Lidifen, Lidifen-f, Lobufen, M01AE01, Mandafen, Manorfen, Maxa-gesic, Medipren, Midol, Midol liquid gels, Migrafen, Motrin, Motrin ib, Motrin migraine pain, Novaprin, NSC-256857, Nuprin, Nurofen 400, Nurofen back pain sr, Nurofen child, Nurofen for child, Nurofen plus, Nurofen recovery, Nurofen sinus, Nurofen solb, Orbifen, Pacifene, Paxofen, Pedea, Pediatric advil, Phensic, Phor pain, Phorpain, Phorpain max strgh, Profen, Proflex, Proflex sr, RD 13621, Relcofen, Rimafen, Rufen, Seclodin, Suspren, Tab-profen, U-18,573, U-18573)
AKR1C3/Aldo-keto-reductase family 1 member C3 in Human [ChEMBL: CHEMBL4681] [GtoPdb: 1382] [UniProtKB: P42330] There should be some charts here, you may need to enable JavaScript!
5-LOX/Arachidonate 5-lipoxygenase in Human [ChEMBL: CHEMBL215] [GtoPdb: 1385] [UniProtKB: P09917] 5-LOX/Arachidonate 5-lipoxygenase in Rat [ChEMBL: CHEMBL312] [GtoPdb: 1385] [UniProtKB: P12527] There should be some charts here, you may need to enable JavaScript!
COX-1 /Cyclooxygenase-1 in Human [ChEMBL: CHEMBL221] [GtoPdb: 1375] [UniProtKB: P23219] Cyclooxygenase-1 in Bovine [ChEMBL: CHEMBL2860] [UniProtKB: O62664] Cyclooxygenase-1 in Sheep [ChEMBL: CHEMBL2949] [UniProtKB: P05979] There should be some charts here, you may need to enable JavaScript!
COX-2 /Cyclooxygenase-2 in Human [ChEMBL: CHEMBL230] [GtoPdb: 1376] [UniProtKB: P35354] Cyclooxygenase-2 in Bovine [ChEMBL: CHEMBL3331] [UniProtKB: O62698] Cyclooxygenase-2 in Sheep [ChEMBL: CHEMBL4102] [UniProtKB: P79208] COX-2 /Cyclooxygenase-2 in Mouse [ChEMBL: CHEMBL4321] [GtoPdb: 1376] [UniProtKB: Q05769] There should be some charts here, you may need to enable JavaScript!
Dehydrogenase/reductase SDR family member 9 in Rat [ChEMBL: CHEMBL1075220] [UniProtKB: Q8VD48] There should be some charts here, you may need to enable JavaScript!
epidermal growth factor receptor/Epidermal growth factor receptor erbB1 in Human [ChEMBL: CHEMBL203] [GtoPdb: 1797] [UniProtKB: P00533] There should be some charts here, you may need to enable JavaScript!
fatty acid binding protein 1/Fatty acid-binding protein, liver in Rat [ChEMBL: CHEMBL5738] [GtoPdb: 2531] [UniProtKB: P02692] There should be some charts here, you may need to enable JavaScript!
L-PGDS/Prostaglandin-H2 D-isomerase in Mouse [ChEMBL: CHEMBL4334] [GtoPdb: 1380] [UniProtKB: O09114] There should be some charts here, you may need to enable JavaScript!
Serum albumin in Human [ChEMBL: CHEMBL3253] [UniProtKB: P02768] Serum albumin in Bovine [ChEMBL: CHEMBL3728] [UniProtKB: P02769] There should be some charts here, you may need to enable JavaScript!
OAT6/Solute carrier family 22 member 20 in Mouse [ChEMBL: CHEMBL5269] [GtoPdb: 1037] [UniProtKB: Q80UJ1] There should be some charts here, you may need to enable JavaScript!
Organic anion transporter 1/Solute carrier family 22 member 6 in Human [ChEMBL: CHEMBL1641347] [GtoPdb: 1025] [UniProtKB: Q4U2R8] Organic anion transporter 1/Solute carrier family 22 member 6 in Mouse [ChEMBL: CHEMBL5653] [GtoPdb: 1025] [UniProtKB: Q8VC69] Organic anion transporter 1/Solute carrier family 22 member 6 in Rat [ChEMBL: CHEMBL1777665] [GtoPdb: 1025] [UniProtKB: O35956] There should be some charts here, you may need to enable JavaScript!
ASIC1 in Human [GtoPdb: 684] [UniProtKB: P78348] There should be some charts here, you may need to enable JavaScript!
SMCT1 in Human [GtoPdb: 922] [UniProtKB: Q8N695] There should be some charts here, you may need to enable JavaScript!

DB	Assay description	Assay Type	Standard value	Standard parameter	Original value	Original units	Original parameter	Reference
AKR1C3/Aldo-keto-reductase family 1 member C3 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4681] [GtoPdb: 1382] [UniProtKB: P42330]
ChEMBL	Inhibition of recombinant human AKR1C3	B	4.48	pIC50	33000	nM	IC50	Bioorg Med Chem Lett (2005) 15: 5170-5175 [PMID:16183274]
ChEMBL	Inhibition of AKR1C3 (unknown origin)	B	5	pIC50	10000	nM	IC50	Medchemcomm (2018) 9: 969-981 [PMID:30108986]
5-LOX/Arachidonate 5-lipoxygenase in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL215] [GtoPdb: 1385] [UniProtKB: P09917]
ChEMBL	Inhibitory activity against 5-lipoxygenase in Human whole blood (HWBL) stimulated with calcium ionophore (A23187) and LTB4 measured by enzyme immunoassay	F	4.77	pIC50	17000	nM	IC50	J Med Chem (1997) 40: 819-824 [PMID:9057869]
5-LOX/Arachidonate 5-lipoxygenase in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL312] [GtoPdb: 1385] [UniProtKB: P12527]
ChEMBL	Inhibition of 5-LOX in RBL1 cells assessed as reduction in LTB4 production using A23187-induced arachidonic acid as substrate preincubated for 2 hrs followed by A23187 addition measured after 15 mins by LC-MS/MS analysis	B	4	pIC50	>100000	nM	IC50	ACS Med Chem Lett (2017) 8: 864-868 [PMID:28835803]
ChEMBL	Inhibitory activity against 5-lipoxygenase catalysis (5-LO) in sonicated rat basophilic leukemia cell lysate	F	4.85	pIC50	14000	nM	IC50	J Med Chem (1997) 40: 819-824 [PMID:9057869]
COX-1 /Cyclooxygenase-1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL221] [GtoPdb: 1375] [UniProtKB: P23219]
ChEMBL	Inhibition of human recombinant COX1 expressed in Sf9 cells using arachidonic acid and ADHP incubated for 3 mins by fluorimetry	B	4.47	pIC50	34000	nM	IC50	J Med Chem (2020) 63: 12614-12622 [PMID:32931274]
ChEMBL	Inhibition of PGHS1 assessed as conversion of arachidonic acid to prostaglandin	B	4.6	pIC50	25000	nM	IC50	J Nat Prod (1999) 62: 294-296 [PMID:10075763]
ChEMBL	Inhibition of COX1-catalyzed prostaglandin biosynthesis 10 mins of preincubation	B	4.74	pIC50	18000	nM	IC50	J Nat Prod (1998) 61: 1212-1215 [PMID:9784154]
ChEMBL	Inhibition activity against recombinant human Prostaglandin G/H synthase 1	B	4.95	pIC50	11100	nM	IC50	J Med Chem (1997) 40: 819-824 [PMID:9057869]
ChEMBL	Inhibition of COX1 in human whole blood after 15 mins	B	5.16	pIC50	6900	nM	IC50	Bioorg Med Chem (2007) 15: 4876-4890 [PMID:17517512]
ChEMBL	Compound was evaluated for inhibition of Prostaglandin G/H synthase 1	B	5.2	pIC50	6300	nM	IC50	Bioorg Med Chem Lett (1996) 6: 707-712
GtoPdb	-	-	5.52	pIC50	3000	nM	IC50	J Med Chem (1998) 41: 1124-37 [PMID:9544212]
ChEMBL	Inhibition of Prostaglandin G/H synthase 1 (COX-1).	B	5.52	pIC50	3000	nM	IC50	J Med Chem (1998) 41: 1124-1137 [PMID:9544212]
ChEMBL	Concentration (in uM) to inhibit 50% of Prostaglandin G/H synthase 1 (COX-1) and is expressed in IC50.	B	5.52	pIC50	3000	nM	IC50	J Med Chem (1998) 41: 1112-1123 [PMID:9544211]
ChEMBL	Inhibitory activity against prostaglandin G/H synthase 1 (COX-1)	B	5.52	pIC50	3000	nM	IC50	J Med Chem (1998) 41: 3515-3529 [PMID:9719605]
ChEMBL	Inhibition of COX-1 (unknown origin) using arachidonic acid as substrate measured after 10 mins by fluorometric based multimode microplate reader	B	5.57	pIC50	2704	nM	IC50	Eur J Med Chem (2021) 209: 112918-112918 [PMID:33071054]
ChEMBL	Inhibition of COX-1 in human washed platelet assessed as inhibition of 10 uM arachidonic acid-induced TXB2 formation incubated for 25 mins prior to arachidonic acid challenge measured after 30 mins by radioimmunoassay	B	5.73	pIC50	1860	nM	IC50	J Med Chem (2013) 56: 4277-4299 [PMID:23651359]
ChEMBL	Inhibition of COX-1 in human washed platelet assessed as inhibition of 0.5 uM arachidonic acid-induced TXB2 formation incubated for 25 mins prior to arachidonic acid challenge measured after 30 mins by radioimmunoassay	B	6.54	pIC50	290	nM	IC50	J Med Chem (2013) 56: 4277-4299 [PMID:23651359]
ChEMBL	DRUGMATRIX: Cyclooxygenase COX-1 enzyme inhibition (substrate: Arachidonic acid)	B	6.67	pIC50	212	nM	IC50	DrugMatrix in vitro pharmacology data
Cyclooxygenase-1 in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2860] [UniProtKB: O62664]
ChEMBL	Inhibition of bovine seminal microsomal COX1 assessed as PGE2 production preincubated for 10 mins	B	4.74	pIC50	18000	nM	IC50	J Nat Prod (1998) 61: 2-7 [PMID:9461646]
ChEMBL	Inhibition of bovine seminal microsomal COX1 assessed as PGE2 production	B	4.89	pIC50	13000	nM	IC50	J Nat Prod (1998) 61: 2-7 [PMID:9461646]
ChEMBL	Inhibition of bovine COX1 by enzyme immunoassay	B	5.54	pIC50	2900	nM	IC50	J Med Chem (2009) 52: 1525-1529 [PMID:19296694]
ChEMBL	Inhibition of bovine COX-1 by enzyme immuno assay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2009) 19: 6855-6861 [PMID:19884005]
Cyclooxygenase-1 in Sheep (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL2949] [UniProtKB: P05979]
ChEMBL	Inhibition of sheep placental cotyledons COX1	B	5.05	pKi	9000	nM	Ki	J Med Chem (2011) 54: 8555-8562 [PMID:22091869]
ChEMBL	Inhibition of ovine COX1 using arachidonic acid as substrate pretreated for 3 mins followed by substrate addition measured immediately	B	4.14	pIC50	72800	nM	IC50	J Nat Prod (2017) 80: 2472-2477 [PMID:28885836]
ChEMBL	Competitive inhibition of ovine COX-1 assessed as reduction in PGE2 production using 6250 nM arachidonic acid as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured after 20 mins by ELISA	B	4.15	pIC50	70100	nM	IC50	ACS Med Chem Lett (2021) 12: 610-616 [PMID:33854702]
ChEMBL	Inhibition of ovine COX-1 assessed as decrease in PGH2 production using arachidonic acid as substrate treated with enzyme for 10 mins prior to substrate challenge for 2 mins by enzyme immunoassay	B	4.59	pIC50	26000	nM	IC50	Bioorg Med Chem Lett (2014) 24: 1958-1962 [PMID:24656662]
ChEMBL	Inhibitory concentration against Prostaglandin G/H synthase 1 of ram seminal vesicles at 100 uM	B	4.84	pIC50	14400	nM	IC50	Bioorg Med Chem Lett (2005) 15: 1793-1797 [PMID:15780608]
ChEMBL	Inhibition of ovine COX-1 assessed as residual activity by measuring formation of 12-HHT from arachidonic acid by HPLC analysis	B	4.92	pIC50	12000	nM	IC50	Bioorg Med Chem (2011) 19: 5372-5382 [PMID:21873070]
ChEMBL	Inhibition of ovine COX1 after 5 mins	B	4.92	pIC50	12000	nM	IC50	J Med Chem (2010) 53: 4691-4700 [PMID:20503989]
ChEMBL	Inhibition of purified ovine COX1 pre-treated for 1 hr before 10-acetyl-3,7-dihydroxyphenoxazin substrate addition in absence of porcine liver esterase by fluorescence assay	B	5	pIC50	10100	nM	IC50	ACS Med Chem Lett (2014) 5: 983-988 [PMID:25221653]
ChEMBL	Inhibition of Ovine COX-1 preincubated for 15 mins by fluorescence analysis	B	5	pIC50	>10000	nM	IC50	ACS Med Chem Lett (2015) 6: 1086-1090 [PMID:26487917]
ChEMBL	Tested for inhibitory activity against Prostaglandin G/H synthase 1 from ovine	B	5.05	pIC50	9000	nM	IC50	J Med Chem (1995) 38: 3895-3901 [PMID:7562922]
ChEMBL	Inhibition of ram seminal vesicle COX1 assessed as reduction in PGE2 formation pre-incubated for 5 mins before arachidonic acid addition and measured after 20 mins by ELISA	B	5.2	pIC50	6300	nM	IC50	J Nat Prod (2017) 80: 999-1006 [PMID:28322565]
ChEMBL	Inhibition of ovine COX1 pre-incubated for 10 mins before arachidonic acid substrate addition by enzyme immunoassay	B	5.3	pIC50	5000	nM	IC50	J Med Chem (2012) 55: 8807-8826 [PMID:23043222]
ChEMBL	Inhibition of ovine COX1 using arachidonic acid as substrate pretreated for 5 mins followed by substrate addition and measured after 2 mins by fluorescence based enzyme immunoassay	B	5.39	pIC50	4120	nM	IC50	Eur J Med Chem (2019) 180: 86-98 [PMID:31301566]
ChEMBL	Inhibition of ovine COX-1	B	5.4	pIC50	4000	nM	IC50	J Med Chem (2011) 54: 1356-1364 [PMID:21280601]
ChEMBL	Competitive inhibition of ovine COX-1 assessed as reduction in PGE2 production using 250 nM arachidonic acid as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured after 20 mins by ELISA	B	5.4	pIC50	4000	nM	IC50	ACS Med Chem Lett (2021) 12: 610-616 [PMID:33854702]
ChEMBL	Inhibition of ovine COX1 by enzyme immuno assay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2011) 21: 6074-6080 [PMID:21890358]
ChEMBL	Inhibition of ovine COX1	B	5.54	pIC50	2900	nM	IC50	J Med Chem (2012) 55: 688-696 [PMID:22148253]
ChEMBL	Inhibition of ovine COX1	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2013) 23: 163-168 [PMID:23200247]
ChEMBL	Inhibition of ovine COX1 by enzyme immuno assay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem (2007) 15: 4767-4774 [PMID:17509888]
ChEMBL	Inhibition of ovine COX1 by enzyme immuno assay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2016) 26: 406-412 [PMID:26691756]
ChEMBL	In vitro inhibitory concentration against COX-1 enzyme	B	5.54	pIC50	2900	nM	IC50	J Med Chem (2005) 48: 4061-4067 [PMID:15943479]
ChEMBL	Inhibition of ovine COX1 by enzyme immuno assay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem (2008) 16: 9694-9698 [PMID:18930406]
ChEMBL	Inhibition of ovine COX1 by enzyme immunoassay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2009) 19: 3014-3018 [PMID:19419861]
ChEMBL	Inhibition of ovine COX1 by enzyme immunoassay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem (2009) 17: 5182-5188 [PMID:19500994]
ChEMBL	Inhibition of ovine COX1 by enzyme immunoassay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2010) 20: 1324-1329 [PMID:20097072]
ChEMBL	Inhibition of ovine COX1 by enzyme immuno assay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2010) 20: 896-902 [PMID:20045320]
ChEMBL	Inhibition of ovine COX1 by enzyme immuno assay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2010) 20: 2168-2173 [PMID:20202839]
ChEMBL	Inhibition of sheep COX1 by enzyme immunoassay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2010) 20: 4544-4549 [PMID:20576432]
ChEMBL	Inhibition of ovine COX1 by enzyme immuno assay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2011) 21: 3951-3956 [PMID:21641217]
ChEMBL	Inhibition of ovine COX1 by enzyme immuno assay	B	5.54	pIC50	2900	nM	IC50	Bioorg Med Chem Lett (2009) 19: 584-588 [PMID:19136259]
ChEMBL	Inhibition of ovine COX-1 assessed as reduction in PGE2 production using 10 uM arachidonic acid as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured after 20 mins by ELISA	B	5.66	pIC50	2190	nM	IC50	ACS Med Chem Lett (2021) 12: 610-616 [PMID:33854702]
ChEMBL	Inhibition of ovine COX1 assessed as reduction in Prostaglandin production by enzyme immunoassay	B	5.85	pIC50	1420	nM	IC50	Bioorg Med Chem (2018) 26: 891-902 [PMID:29373271]
ChEMBL	Reversible competitive inhibition of prostaglandin G/H synthase 1	B	5.97	pIC50	1060	nM	IC50	Bioorg Med Chem Lett (2004) 14: 667-671 [PMID:14741265]
COX-2 /Cyclooxygenase-2 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL230] [GtoPdb: 1376] [UniProtKB: P35354]
ChEMBL	Inhibition of human recombinant COX-2 assessed as residual activity by measuring formation of 12-HHT from arachidonic acid by HPLC analysis	B	4.1	pIC50	80000	nM	IC50	Bioorg Med Chem (2011) 19: 5372-5382 [PMID:21873070]
ChEMBL	Inhibition of human recombinant COX2 after 5 mins	B	4.1	pIC50	80000	nM	IC50	J Med Chem (2010) 53: 4691-4700 [PMID:20503989]
ChEMBL	Inhibition of human COX2 pre-incubated for 10 mins before substrate addition by enzyme immunoassay	B	4.44	pIC50	36000	nM	IC50	J Med Chem (2012) 55: 8807-8826 [PMID:23043222]
ChEMBL	Inhibitory activity against prostaglandin G/H synthase 2 (COX-2)	B	4.52	pIC50	30000	nM	IC50	J Med Chem (1998) 41: 3515-3529 [PMID:9719605]
ChEMBL	Concentration (in uM) to inhibit 50% of Prostaglandin G/H synthase 2 (COX-2) and is expressed in IC50.	B	4.52	pIC50	30000	nM	IC50	J Med Chem (1998) 41: 1112-1123 [PMID:9544211]
ChEMBL	Inhibition of Prostaglandin G/H synthase 2 (COX-2).	B	4.52	pIC50	30000	nM	IC50	J Med Chem (1998) 41: 1124-1137 [PMID:9544212]
ChEMBL	Inhibition of human recombinant COX-2 assessed as decrease in PGH2 production using arachidonic acid as substrate treated with enzyme for 10 mins prior to substrate challenge for 2 mins by enzyme immunoassay	B	4.52	pIC50	30000	nM	IC50	Bioorg Med Chem Lett (2014) 24: 1958-1962 [PMID:24656662]
ChEMBL	DRUGMATRIX: Cyclooxygenase COX-2 enzyme inhibition (substrate: Arachidonic acid)	B	4.59	pIC50	25530	nM	IC50	DrugMatrix in vitro pharmacology data
ChEMBL	Inhibition of COX2 (unknown origin)	B	4.84	pIC50	14500	nM	IC50	Eur J Med Chem (2017) 138: 1002-1033 [PMID:28759875]
ChEMBL	Inhibition of recombinant human COX2 using arachidonic acid as substrate pretreated for 5 mins followed by substrate addition and measured after 2 mins by fluorescence based enzyme immunoassay	B	5.05	pIC50	8910	nM	IC50	Eur J Med Chem (2019) 180: 86-98 [PMID:31301566]
ChEMBL	Tested for inhibitory activity against Prostaglandin G/H synthase 2 from human	B	5.1	pIC50	8000	nM	IC50	J Med Chem (1995) 38: 3895-3901 [PMID:7562922]
ChEMBL	Inhibition of COX2 in human whole blood after 15 mins	B	5.21	pIC50	6200	nM	IC50	Bioorg Med Chem (2007) 15: 4876-4890 [PMID:17517512]
ChEMBL	Inhibition of COX-2 (unknown origin) using arachidonic acid as substrate incubated for 5 to 10 mins by fluorometric analysis	B	5.27	pIC50	5330	nM	IC50	RSC Med Chem (2022) 13: 471-496 [PMID:35685617]
ChEMBL	Inhibition of COX-2 (unknown origin) using arachidonic acid as substrate measured after 10 mins by fluorometric based multimode microplate reader	B	5.27	pIC50	5326	nM	IC50	Eur J Med Chem (2021) 209: 112918-112918 [PMID:33071054]
ChEMBL	Inhibition of human recombinant COX2 pre-treated for 1 hr before 10-acetyl-3,7-dihydroxyphenoxazin substrate addition in absence of porcine liver esterase by fluorescence assay	B	5.37	pIC50	4300	nM	IC50	ACS Med Chem Lett (2014) 5: 983-988 [PMID:25221653]
ChEMBL	Inhibition of human recombinant COX2 assessed as reduction in PGE2 formation pre-incubated for 5 mins before arachidonic acid addition and measured after 20 mins by ELISA	B	5.38	pIC50	4200	nM	IC50	J Nat Prod (2017) 80: 999-1006 [PMID:28322565]
ChEMBL	Inhibition of human recombinant COX2 expressed in Sf9 cells using arachidonic acid and ADHP incubated for 5 mins by fluorimetry	B	5.48	pIC50	3300	nM	IC50	J Med Chem (2020) 63: 12614-12622 [PMID:32931274]
ChEMBL	Inhibition of human recombinant COX-2 assessed as reduction in PGE2 production using 10 uM arachidonic acid as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured after 20 mins by ELISA	B	5.48	pIC50	3300	nM	IC50	ACS Med Chem Lett (2021) 12: 610-616 [PMID:33854702]
ChEMBL	Inhibition of human recombinant COX2 assessed as reduction in PGE2 production using arachidonic acid substrate by ELISA	B	5.7	pIC50	2000	nM	IC50	J Nat Prod (2019) 82: 1839-1848 [PMID:31268709]
ChEMBL	Inhibition of COX2 (unknown origin) assessed as reduction in PGF2alpha content using arachidonic acid as substrate preincubated for 10 mins followed by substrate addition and measured after 2 mins by enzyme immunoassay	B	5.75	pIC50	1790	nM	IC50	J Med Chem (2022) 65: 14916-14937 [PMID:36282007]
ChEMBL	Inhibition of human recombinant COX-2	B	5.85	pIC50	1400	nM	IC50	J Med Chem (2011) 54: 1356-1364 [PMID:21280601]
ChEMBL	Inhibition of human COX2 expressed in baculovirus system	B	5.87	pIC50	1350	nM	IC50	J Med Chem (2011) 54: 8555-8562 [PMID:22091869]
GtoPdb	-	-	5.87	pIC50	1350	nM	IC50	J Med Chem (2011) 54: 8555-62 [PMID:22091869]
ChEMBL	Inhibition of human recombinant COX-2 by enzyme immuno assay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2009) 19: 6855-6861 [PMID:19884005]
ChEMBL	Inhibition of human recombinant COX2 by enzyme immuno assay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2011) 21: 3951-3956 [PMID:21641217]
ChEMBL	Inhibition of human COX2 by enzyme immunoassay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2010) 20: 4544-4549 [PMID:20576432]
ChEMBL	Inhibition of human recombinant COX2	B	5.96	pIC50	1100	nM	IC50	J Med Chem (2012) 55: 688-696 [PMID:22148253]
ChEMBL	Inhibition of human recombinant COX2 by enzyme immuno assay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2010) 20: 2168-2173 [PMID:20202839]
ChEMBL	Inhibition of human recombinant COX2	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2013) 23: 163-168 [PMID:23200247]
ChEMBL	Inhibition of human recombinant COX2 by enzyme immunoassay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2010) 20: 1324-1329 [PMID:20097072]
ChEMBL	Inhibition of recombinant human COX-2 preincubated for 15 mins by fluorescence analysis	B	5.96	pIC50	1100	nM	IC50	ACS Med Chem Lett (2015) 6: 1086-1090 [PMID:26487917]
ChEMBL	Inhibition of human recombinant COX2 by enzyme immuno assay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2016) 26: 406-412 [PMID:26691756]
ChEMBL	Inhibition of human recombinant COX2 by enzyme immunoassay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem (2009) 17: 5182-5188 [PMID:19500994]
ChEMBL	Inhibition of recombinant human N-terminal His-tagged COX2 expressed in baculovirus infected Sf21 cells assessed as reduction in prostaglandin production by enzyme immunoassay	B	5.97	pIC50	1080	nM	IC50	Bioorg Med Chem (2018) 26: 891-902 [PMID:29373271]
ChEMBL	Inhibition activity against recombinant human Prostaglandin G/H synthase 2	B	7	pIC50	100	nM	IC50	J Med Chem (1997) 40: 819-824 [PMID:9057869]
Cyclooxygenase-2 in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3331] [UniProtKB: O62698]
ChEMBL	Inhibition of bovine COX2 by enzyme immunoassay	B	5.96	pIC50	1100	nM	IC50	J Med Chem (2009) 52: 1525-1529 [PMID:19296694]
Cyclooxygenase-2 in Sheep (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4102] [UniProtKB: P79208]
ChEMBL	Inhibition of sheep placental cotyledons COX2	B	5.05	pKi	9000	nM	Ki	J Med Chem (2011) 54: 8555-8562 [PMID:22091869]
ChEMBL	Inhibition of ovine COX2 assessed as reduction in PGH2 production by enzyme immunoassay	B	5.85	pIC50	1400	nM	IC50	Bioorg Med Chem (2018) 26: 4113-4126 [PMID:29980364]
ChEMBL	Inhibition of ovine COX2 by enzyme immuno assay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem (2007) 15: 4767-4774 [PMID:17509888]
ChEMBL	Inhibition of ovine COX2 by enzyme immuno assay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem (2008) 16: 9694-9698 [PMID:18930406]
ChEMBL	Inhibition of ovine COX2 by enzyme immuno assay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2009) 19: 584-588 [PMID:19136259]
ChEMBL	In vitro inhibitory concentration against COX-2 enzyme	B	5.96	pIC50	1100	nM	IC50	J Med Chem (2005) 48: 4061-4067 [PMID:15943479]
ChEMBL	Inhibition of ovine COX2 by enzyme immuno assay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2010) 20: 896-902 [PMID:20045320]
ChEMBL	Inhibition of ovine COX2 by enzyme immuno assay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2011) 21: 6074-6080 [PMID:21890358]
ChEMBL	Inhibition of ovine COX2 by enzyme immunoassay	B	5.96	pIC50	1100	nM	IC50	Bioorg Med Chem Lett (2009) 19: 3014-3018 [PMID:19419861]
COX-2 /Cyclooxygenase-2 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4321] [GtoPdb: 1376] [UniProtKB: Q05769]
ChEMBL	Inhibition of COX-2 in mouse RAW264.7 cells assessed as decrease in LPS-induced PGE2 production treated prior to LPS challenge by enzyme immunoassay	B	6.07	pIC50	860	nM	IC50	Bioorg Med Chem Lett (2014) 24: 1958-1962 [PMID:24656662]
Dehydrogenase/reductase SDR family member 9 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1075220] [UniProtKB: Q8VD48]
ChEMBL	Inhibition of rat liver 3-alpha-HSD assessed as 5-beta-dihydrocortisone reduction	B	4	pIC50	100000	nM	IC50	Bioorg Med Chem (2007) 15: 4876-4890 [PMID:17517512]
epidermal growth factor receptor/Epidermal growth factor receptor erbB1 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL203] [GtoPdb: 1797] [UniProtKB: P00533]
ChEMBL	Inhibition of EGFR (unknown origin) using tyrosine 4 as substrate by fluorescence analysis	B	5	pIC50	>10000	nM	IC50	ACS Med Chem Lett (2015) 6: 1086-1090 [PMID:26487917]
fatty acid binding protein 1/Fatty acid-binding protein, liver in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5738] [GtoPdb: 2531] [UniProtKB: P02692]
ChEMBL	Displacement of 1-anilinonaphthalene-8-sulphonic acid from rat recombinant L-FABP high binding affinity site expressed in Escherichia coli BL21 by competitive fluorescence displacement assay	B	4.32	pKi	47600	nM	Ki	J Med Chem (2008) 51: 3755-3764 [PMID:18533710]
L-PGDS/Prostaglandin-H2 D-isomerase in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL4334] [GtoPdb: 1380] [UniProtKB: O09114]
ChEMBL	Concentration required to inhibit 50% activity of prostaglandin synthetase was determined in vitro in mouse brain microsomes	B	4.89	pIC50	13000	nM	IC50	J Med Chem (1991) 34: 1099-1110 [PMID:1900533]
Serum albumin in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3253] [UniProtKB: P02768]
ChEMBL	Binding affinity to human serum albumin assessed as dissociation constant and measured after 5 mins by MST assay	B	4.14	pKd	73000	nM	Kd	J Med Chem (2022) 65: 710-733 [PMID:34939412]
Serum albumin in Bovine (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL3728] [UniProtKB: P02769]
ChEMBL	Binding affinity to bovine serum albumin	B	5.52	pIC50	3000	nM	IC50	Bioorg Med Chem (2008) 16: 1162-1173 [PMID:18024138]
OAT6/Solute carrier family 22 member 20 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5269] [GtoPdb: 1037] [UniProtKB: Q80UJ1]
ChEMBL	Inhibition of mouse Oat6-mediated [3H]ES uptake in Xenopus oocytes after 1 hr	B	5.95	pKi	1122.02	nM	Ki	J Biol Chem (2007) 282: 23841-23853 [PMID:17553798]
ChEMBL	Inhibition of mouse Oat6-mediated [3H]ES uptake in Xenopus oocytes after 1 hr	B	5.96	pKi	1100	nM	Ki	J Biol Chem (2007) 282: 23841-23853 [PMID:17553798]
Organic anion transporter 1/Solute carrier family 22 member 6 in Human (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1641347] [GtoPdb: 1025] [UniProtKB: Q4U2R8]
ChEMBL	TP_TRANSPORTER: inhibition of Adefovir uptake in OAT1-expressing CHO cells	F	5.1	pIC50	8000	nM	IC50	J Pharmacol Exp Ther (2000) 295: 10-15 [PMID:10991954]
Organic anion transporter 1/Solute carrier family 22 member 6 in Mouse (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL5653] [GtoPdb: 1025] [UniProtKB: Q8VC69]
ChEMBL	Inhibition of mouse Oat1-mediated [3H]PAH uptake in Xenopus oocytes after 1 hr	B	5.33	pKi	4700	nM	Ki	J Biol Chem (2007) 282: 23841-23853 [PMID:17553798]
ChEMBL	Inhibition of mouse Oat1-mediated [3H]PAH uptake in Xenopus oocytes after 1 hr	B	5.33	pKi	4677.35	nM	Ki	J Biol Chem (2007) 282: 23841-23853 [PMID:17553798]
Organic anion transporter 1/Solute carrier family 22 member 6 in Rat (target type: SINGLE PROTEIN) [ChEMBL: CHEMBL1777665] [GtoPdb: 1025] [UniProtKB: O35956]
ChEMBL	TP_TRANSPORTER: inhibition of PAH uptake in Xenopus laevis oocytes	F	5.46	pKi	3500	nM	Ki	Mol Pharmacol (1999) 55: 847-854 [PMID:10220563]
ASIC1 in Human [GtoPdb: 684] [UniProtKB: P78348]
GtoPdb	-	-	3.5	pIC50	~350000	nM	IC50	J Neurosci (2001) 21: 8026-33 [PMID:11588175]; J Med Chem (2017) 60: 8192-8200 [PMID:28949138]
SMCT1 in Human [GtoPdb: 922] [UniProtKB: Q8N695]
GtoPdb	-	-	4.2	pIC50	63095	nM	IC50	Pharm Res (2006) 23: 1209-16 [PMID:16729224]

ChEMBL data shown on this page come from version 34:

Zdrazil B, Felix E, Hunter F, Manners EJ, Blackshaw J, Corbett S, de Veij M, Ioannidis H, Lopez DM, Mosquera JF, Magarinos MP, Bosc N, Arcila R, Kizilören T, Gaulton A, Bento AP, Adasme MF, Monecke P, Landrum GA, Leach AR. (2024). The ChEMBL Database in 2023: a drug discovery platform spanning multiple bioactivity data types and time periods. Nucleic Acids Res., 52(D1). DOI: 10.1093/nar/gkad1004. [EPMCID:10767899] [PMID:37933841]
Davies M, Nowotka M, Papadatos G, Dedman N, Gaulton A, Atkinson F, Bellis L, Overington JP. (2015) 'ChEMBL web services: streamlining access to drug discovery data and utilities.' Nucleic Acids Res., 43(W1). DOI: 10.1093/nar/gkv352. [EPMCID:25883136]